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Immunoproteomic analysis of capsulate and non-capsulate strains of Lactococcus garvieae
DC Field | Value | Language |
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dc.contributor.author | Shin, G. W. | - |
dc.contributor.author | Palaksha, K. J. | - |
dc.contributor.author | Kim, Y. R. | - |
dc.contributor.author | Nho, S. W. | - |
dc.contributor.author | Cho, J. H. | - |
dc.contributor.author | Heo, N. E. | - |
dc.contributor.author | Heo, G. J. | - |
dc.contributor.author | Park, S. C. | - |
dc.contributor.author | Jung, T. S. | - |
dc.date.accessioned | 2023-05-30T07:06:04Z | - |
dc.date.available | 2023-05-30T07:06:04Z | - |
dc.date.created | 2021-04-15 | - |
dc.date.created | 2021-04-15 | - |
dc.date.created | 2021-04-15 | - |
dc.date.issued | 2007-01 | - |
dc.identifier.citation | Veterinary Microbiology, Vol.119 No.2-4, pp.205-212 | - |
dc.identifier.issn | 0378-1135 | - |
dc.identifier.uri | https://hdl.handle.net/10371/192559 | - |
dc.description.abstract | A comparative immunoproteomic study was carried out to investigate the immunogenicity of capsulate (KG9408) and noncapsulate (NSS9310) strains of Lactococcus garvieae. Immumoblot assays, following two-dimensional gel electrophoresis (2-DE) for L. garvieae strains, revealed a significant difference between anti-capsulate and anti-non-capsulate rabbit sera with respect to the number and antigenicity of antigenic spots. Anti-capsulate and anti-non-capsulate rabbit sera reacted with an average of 72 and 127 antigenic spots, respectively. The strong reaction of anti-non-capsulate sera with elongation factor (EF)-G and -Tu, and GMP synthase, of the L. garvieae strains identifies these as specific major antigens. This study clearly demonstrates the differences in 2-DE immunoblot profiles between the capsulate and non-capsulate strains of L. garvieae. These differences may be the reason for variations in immunogenicity between capsulate and non-capsulate strains. Glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, arginine deaminase and ornithine carbamoyltransferase were identified from the 2-DE inummoblot profiles of both strains. Therefore, these common antigens are potential markers for the development of vaccines against L. garvieae, irrespective of strain. Immunoproteomics, a powerful tool for studying antigens at the proteomic level, allowed a comparative investigation of the immunogenicity of capsulate and non-capsulate strains of L. garvieae for vaccine development. (c) 2006 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Immunoproteomic analysis of capsulate and non-capsulate strains of Lactococcus garvieae | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.vetmic.2006.08.021 | - |
dc.citation.journaltitle | Veterinary Microbiology | - |
dc.identifier.wosid | 000243870300012 | - |
dc.identifier.scopusid | 2-s2.0-33845944897 | - |
dc.citation.endpage | 212 | - |
dc.citation.number | 2-4 | - |
dc.citation.startpage | 205 | - |
dc.citation.volume | 119 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, S. C. | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | YELLOWTAIL SERIOLA-QUINQUERADIATA | - |
dc.subject.keywordPlus | FLOUNDER PARALICHTHYS-OLIVACEUS | - |
dc.subject.keywordPlus | TROUT ONCORHYNCHUS-MYKISS | - |
dc.subject.keywordPlus | RAINBOW-TROUT | - |
dc.subject.keywordPlus | FISH PATHOGEN | - |
dc.subject.keywordPlus | CELL-SURFACE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | ENDOCARDITIS | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordAuthor | Lactococcus garvieae | - |
dc.subject.keywordAuthor | immunoproteomics | - |
dc.subject.keywordAuthor | capsule | - |
dc.subject.keywordAuthor | antigenic profiles | - |
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