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A new 3D reconstituted human corneal epithelium model as an alternative method for the eye irritation test
Cited 42 time in
Web of Science
Cited 45 time in Scopus
- Authors
- Issue Date
- 2011-02
- Publisher
- Elsevier BV
- Citation
- Toxicology in Vitro, Vol.25 No.1, pp.403-410
- Abstract
- Many efforts are being made to develop new alternative in vitro test methods for the eye irritation test. Here we report a new reconstructed human corneal epithelial model (MCTT HCE model) prepared from primary-cultured human limbal epithelial cells as a new alternative in vitro eye irritation test method. In histological and immunohistochemical observation, MCTT HCE model displayed a morphology and bio-marker expressions similar to intact human cornea. Moreover, the barrier function was well preserved as measured by high transepithelial electrical resistance, effective time-50 for Triton X-100, and corneal thickness. To employ the model as a new alternative method for eye irritation test, protocol refinement was performed and optimum assay condition was determined including treatment time, treatment volume, post-incubation time and rinsing method. Using the refined protocol, 25 reference chemicals with known eye irritation potentials were tested. With the viability cut-off value at 50%, chemicals were classified to irritant or non-irritant. When compared with GHS classification, the MCTT HCE model showed the accuracy of 88%, sensitivity of 100% and specificity of 77%. These results suggest that the MCTT HCE model might be useful as a new alternative eye irritation test method. (C) 2010 Elsevier Ltd. All rights reserved.
- ISSN
- 0887-2333
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