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High-resolution open-top axially swept light sheet microscopy
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- Authors
- Issue Date
- 2023-11-08
- Publisher
- BMC
- Citation
- BMC Biology, Vol.21(1):248
- Keywords
- Light sheet microscopy ; Open-top ; Axial swept ; Aberration correction ; Optically cleared tissue
- Abstract
- Background
Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for the high-throughput cellular imaging of optically cleared, large-sized specimens, such as the brain. Despite the development of various OT-LSM techniques, achieving submicron resolution in all dimensions remains.
Results
We developed a high-resolution open-top axially swept LSM (HR-OTAS-LSM) for high-throughput and high-resolution imaging in all dimensions. High axial and lateral resolutions were achieved by using an aberration-corrected axially swept excitation light sheet in the illumination arm and a high numerical aperture (NA) immersion objective lens in the imaging arm, respectively. The high-resolution, high-throughput visualization of neuronal networks in mouse brain and retina specimens validated the performance of HR-OTAS-LSM.
Conclusions
The proposed HR-OTAS-LSM method represents a significant advancement in the high-resolution mapping of cellular networks in biological systems such as the brain and retina.
- ISSN
- 1741-7007
- Language
- English
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