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소 결핵 진단을 위한 인터페론감마 검사 키트의 성능 비교 평가 : Performance comparison and evaluation of interferon-gamma assay kit for bovine tuberculosis diagnosis
DC Field | Value | Language |
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dc.contributor.author | 홍이곤 | - |
dc.contributor.author | 최우재 | - |
dc.contributor.author | 노영혜 | - |
dc.contributor.author | 안선민 | - |
dc.contributor.author | 김은경 | - |
dc.contributor.author | 최은희 | - |
dc.contributor.author | 김단일 | - |
dc.date.accessioned | 2023-12-11T01:07:43Z | - |
dc.date.available | 2023-12-11T01:07:43Z | - |
dc.date.created | 2021-09-02 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.citation | 한국가축위생학회지, Vol.43 No.4, pp.201-209 | - |
dc.identifier.issn | 1225-6552 | - |
dc.identifier.uri | https://hdl.handle.net/10371/197875 | - |
dc.description.abstract | In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon- gamma (IFN-γ) is included in Koreas diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM Ⓡ ) certified by Office International des Epizooties.
In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM Ⓡ and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM Ⓡ , respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM Ⓡ kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TBFeron Plus kit was not inferior to BOVIGAM Ⓡ in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value. | - |
dc.language | 한국어 | - |
dc.publisher | 한국동물위생학회 | - |
dc.title | 소 결핵 진단을 위한 인터페론감마 검사 키트의 성능 비교 평가 | - |
dc.title.alternative | Performance comparison and evaluation of interferon-gamma assay kit for bovine tuberculosis diagnosis | - |
dc.type | Article | - |
dc.identifier.doi | 10.7853/kjvs.2020.43.4.201 | - |
dc.citation.journaltitle | 한국가축위생학회지 | - |
dc.citation.endpage | 209 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 201 | - |
dc.citation.volume | 43 | - |
dc.identifier.kciid | ART002671490 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | 김단일 | - |
dc.description.journalClass | 2 | - |
dc.subject.keywordAuthor | Bovine tuberculosis, Gamma-interferon, Diagnosis, Delayed time, Lymphocyte | - |
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