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Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jun, Bong-Hyun | - |
dc.contributor.author | Kang, Homan | - |
dc.contributor.author | Lee, Yoon-Sik | - |
dc.contributor.author | Jeong, Dae Hong | - |
dc.date.accessioned | 2023-12-11T07:45:49Z | - |
dc.date.available | 2023-12-11T07:45:49Z | - |
dc.date.created | 2020-11-10 | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | Molecules, Vol.17 No.3, pp.2474-2490 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://hdl.handle.net/10371/198642 | - |
dc.description.abstract | Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding. | - |
dc.language | 영어 | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/molecules17032474 | - |
dc.citation.journaltitle | Molecules | - |
dc.identifier.wosid | 000302120600016 | - |
dc.identifier.scopusid | 2-s2.0-84858983400 | - |
dc.citation.endpage | 2490 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 2474 | - |
dc.citation.volume | 17 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Lee, Yoon-Sik | - |
dc.contributor.affiliatedAuthor | Jeong, Dae Hong | - |
dc.type.docType | Review | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ENHANCED RAMAN-SCATTERING | - |
dc.subject.keywordPlus | SPECTROMETRY-BASED PROTEOMICS | - |
dc.subject.keywordPlus | DOPED SILICA NANOPARTICLES | - |
dc.subject.keywordPlus | LINKED-IMMUNOSORBENT-ASSAY | - |
dc.subject.keywordPlus | BEAD-BASED ASSAYS | - |
dc.subject.keywordPlus | FLOW-CYTOMETRY | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | BIOCONJUGATED NANOPARTICLES | - |
dc.subject.keywordPlus | SPECTROSCOPIC TAGS | - |
dc.subject.keywordPlus | MOLECULAR BEACONS | - |
dc.subject.keywordAuthor | optically-encoded bead | - |
dc.subject.keywordAuthor | fluorescence | - |
dc.subject.keywordAuthor | quantum dots | - |
dc.subject.keywordAuthor | surface-enhanced Raman scattering (SERS) | - |
dc.subject.keywordAuthor | bead-based assay | - |
dc.subject.keywordAuthor | label-free detection | - |
dc.subject.keywordAuthor | high-throughput screening | - |
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