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Development of HPLC method for the determination of buspirone in rat plasma using fluorescence detection and its application to a pharmacokinetic study
Cited 10 time in
Web of Science
Cited 11 time in Scopus
- Authors
- Issue Date
- 2016-11
- Publisher
- Pharmaceutical Society of Japan
- Citation
- Chemical and Pharmaceutical Bulletin, Vol.64 No.11, pp.1582-1588
- Abstract
- A simple and sensitive analytical method for the quantitative determination of buspirone in rat plasma by HPLC with fluorescence detection was developed and validated using naproxen as an internal standard. A relatively small-volume (150 mu L) aliquot of rat plasma sample was prepared by a simple deproteinization procedure using acetonitrile as a precipitating organic solvent. Chromatographic separation was performed using Kinetex(R) C8 column with an isocratic mobile phase consisting of acetonitrile and 10-mM potassium phosphate buffer (pH 6.0) at a flow rate of 1.0 mL/min. The eluent was monitored by fluorescence detector at a wavelength pair of 237/380 nm (excitation/emission). The linearity was established at 20.0-5000 ng/mL, and the limit of detection was 6.51 ng/mL. The precision (<= 14.6%), accuracy (89.2-108%), and stability (89.1-101%) were within acceptable ranges. The newly developed method was successfully applied to intravenous and oral pharmacokinetic studies of buspirone in rats.
- ISSN
- 0009-2363
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