Publications
Detailed Information
Determination of manassantin B in rat plasma using a high performance liquid chromatography with fluorescence detection and its quantitative application to pharmacokinetic study
Cited 6 time in
Web of Science
Cited 6 time in Scopus
- Authors
- Issue Date
- 2016-02
- Publisher
- Elsevier BV
- Citation
- Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol.1011, pp.121-127
- Abstract
- A simple, sensitive, rapid, and reproducible analytical method of manassantin B in rat plasma by high performance liquid chromatography with fluorescence detection (HPLC-FL) was developed for its application to pharmacokinetic study in rats. Valsartan (VST) was used as an internal standard (IS) in this quantitative analytical method. Manassantin B and VST were extracted by simple and efficient protein precipitation method. Manassantin B was detected at 282/322 nm (excitation/emission) wavelengths using FL detector. The chromatographic separation was obtained with reverse phase C18 column and the mobile phase composed of potassium phosphate buffer containing 0.025% trifluoroacetic acid (pH 2.5; 5 mM) and acetonitrile including 0.025% trifluoroacetic acid (20:80, v/v) at 1.0 mL/min flow rate. The linearity was established at 25.0-10000 ng/mL and the lower limit of detection (LLOD) was 7 ng/mL. The intra- and inter-day accuracy and precision values of manassantin B were within +/- 15% of the theroretical values and <9% from the nominal concentrations, respectively. Accuracy and precision values of manassantin B after stability tests were also within the acceptable ranges. Developed assay was also successfully applied to pharmacokinetic study after intravenous administration of manassantin B in rats. (C) 2015 Elsevier B.V. All rights reserved.
- ISSN
- 1570-0232
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.