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Whole-Cell Biocatalysis for Producing Ginsenoside Rd from Rb1 Using Lactobacillus rhamnosus GG
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ku, Seockmo | - |
dc.contributor.author | You, Hyun Ju | - |
dc.contributor.author | Park, Myeong Soo | - |
dc.contributor.author | Ji, Geun Eog | - |
dc.date.accessioned | 2024-05-01T01:33:11Z | - |
dc.date.available | 2024-05-01T01:33:11Z | - |
dc.date.created | 2018-09-04 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.citation | Journal of Microbiology and Biotechnology, Vol.26 No.7, pp.1206-1215 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://hdl.handle.net/10371/200142 | - |
dc.description.abstract | Ginsenosides are the major active ingredients in ginseng used for human therapeutic plant medicines. One of the most well-known probiotic bacteria among the various strains on the functional food market is Lactobacillus rhamnosus GG. Biocatalytic methods using probiotic enzymes for producing deglycosylated ginsenosides such as Rd have a growing significance in the functional food industry. The addition of 2% cellobiose (w/v) to glucose-free de Man-Rogosa-Sharpe broths notably induced beta-glucosidase production from L. rhamnosus GG. Enzyme production and activity were optimized at a pH, temperature, and cellobiose concentration of 6.0, 40 degrees C, and 2% (w/v), respectively. Under these controlled conditions, beta-glucosidase production in L. rhamnosus GG was enhanced by 25-fold. Additionally, whole-cell homogenates showed the highest beta-glucosidase activity when compared with disrupted cell suspensions; the cell disruption step significantly decreased the beta-glucosidase activity. Based on the optimized enzyme conditions, whole-cell L. rhamnosus GG was successfully used to convert ginsenoside Rb1 into Rd. | - |
dc.language | 영어 | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Whole-Cell Biocatalysis for Producing Ginsenoside Rd from Rb1 Using Lactobacillus rhamnosus GG | - |
dc.type | Article | - |
dc.identifier.doi | 10.4014/jmb.1601.01002 | - |
dc.citation.journaltitle | Journal of Microbiology and Biotechnology | - |
dc.identifier.wosid | 000385018000006 | - |
dc.identifier.scopusid | 2-s2.0-84982275893 | - |
dc.citation.endpage | 1215 | - |
dc.citation.number | 7 | - |
dc.citation.startpage | 1206 | - |
dc.citation.volume | 26 | - |
dc.identifier.kciid | ART002129629 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | You, Hyun Ju | - |
dc.contributor.affiliatedAuthor | Ji, Geun Eog | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ALPHA-L-ARABINOFURANOSIDASE | - |
dc.subject.keywordPlus | BIFIDOBACTERIUM-LONGUM | - |
dc.subject.keywordPlus | BETA-GLUCOSIDASE | - |
dc.subject.keywordPlus | STRAIN GG | - |
dc.subject.keywordPlus | PROBIOTIC LACTOBACILLI | - |
dc.subject.keywordPlus | ASPERGILLUS-NIDULANS | - |
dc.subject.keywordPlus | PANAX-GINSENG | - |
dc.subject.keywordPlus | L-LYSINE | - |
dc.subject.keywordPlus | BIOTRANSFORMATION | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | Whole-cell bioconversion | - |
dc.subject.keywordAuthor | biocatalysis | - |
dc.subject.keywordAuthor | Lactobacillus rhamnosus | - |
dc.subject.keywordAuthor | ginsenoside | - |
dc.subject.keywordAuthor | beta-glucosidase | - |
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