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DNA hypomethylation of CAGE promotors in squamous cell carcinoma of uterine cervix

Cited 15 time in Web of Science Cited 19 time in Scopus
Authors
Lee, Taek Sang; Kim, Jae Weon; Kang, Gyeong Hoon; Park, Noh Hyun; Song, Yong Sang; Kang, Soon Beom; Lee, Hyo Pyo
Issue Date
2007
Publisher
Wiley-Blackwell
Citation
Ann N Y Acad Sci. 2006;1091:218-224
Keywords
Carcinoma, Squamous Cell/*genetics/metabolismCpG Islands/physiologyDEAD-box RNA Helicases/*genetics/metabolism*DNA MethylationFemaleHumansPolymerase Chain ReactionPromoter Regions, Genetic/*physiologyUterine Cervical Neoplasms/*genetics/metabolism
Abstract
This study was performed to determine whether promotor hypomethylation of CAGE is involved in cervical carcinogenesis. The surgical specimens of 40 cervical squamous cell carcinoma patients treated at Seoul National University Hospital and those of 48 healthy controls were used, with informed consent. We investigated the promotor hypomethylation status of CAGE by methylation-specific polymerase chain reaction (MSP) using primers specific for unmethylated sequences, and found hypomethylation of CAGE promotor at a frequency approaching 90% in cervical squamous cell carcinomas (35/40, 87.5%), but at less than 4% in controls (P < 0.001). This finding provides experimental evidence of the frequent hypomethylation of normally methylated CAGE promotor CpG islands in cervical cancer, and indicates that this hypomethylation is likely to be a valuable surrogate marker for the expression of CAGE. It also provides a clue concerning the molecular mechanisms of carcinogenesis in cervical squamous cell carcinoma.
ISSN
0077-8923 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17341616

http://hdl.handle.net/10371/20047
DOI
https://doi.org/10.1196/annals.1378.068
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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