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Protein-level assembly increases protein sequence recovery from metagenomic samples manyfold
Cited 160 time in
Web of Science
Cited 174 time in Scopus
- Authors
- Issue Date
- 2019-07
- Publisher
- Nature Publishing Group
- Citation
- Nature Methods, Vol.16 No.7, pp.603-609
- Abstract
- The open-source de novo protein-level assembler, Plass (https://plass. mmseqs. com), assembles six-frame-translated sequencing reads into protein sequences. It recovers 2-10 times more protein sequences from complex metagenomes and can assemble huge datasets. We assembled two redundancy-filtered reference protein catalogs, 2 billion sequences from 640 soil samples (soil reference protein catalog) and 292 million sequences from 775 marine eukaryotic metatranscriptomes (marine eukaryotic reference catalog), the largest free collections of protein sequences.
- ISSN
- 1548-7091
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