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MHC expression in a human adult stem cell line and its down-regulation by hCMV US gene transfection

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Authors
Kim, Jae Young; Kim, Donghee; Choi, Inho; Yang, Jae Seok; Lee, Dong-Sup; Lee, Jeong Ryul; Kang, Kyungsun; Kim, Seokhyun; Hwang, Woo Suk; Lee, Jung Sang; Ahn, Curie
Issue Date
2004-09-24
Publisher
Elsevier
Citation
Int J Biochem Cell Biol. 2005 Jan;37(1):69-78.
Keywords
AnimalsAntineoplastic Agents/pharmacologyCell LineCytomegalovirus/*geneticsDown-Regulation/*geneticsHistocompatibility Antigens Class I/*biosynthesis/genetics/immunologyHumansInterferon-gamma/pharmacologyStem Cell TransplantationStem Cells/cytology/immunology/*metabolismTransfectionUp-Regulation/drug effectsViral Proteins/*biosynthesis/genetics
Abstract
Due to their unique capacity to self-renew and for multiple differentiation, stem cells are considered promising candidates for cell replacement therapy in many devastating diseases. However, studies on immune rejection, which is a major problem facing successful stem cell therapy, are rare. In this study, we examined MHC expression in the M13SV1 cell line, which has previously been shown to have stem cell properties and to be non-tumorigenic, in order to determine whether human adult stem cells might be rejected after transplantation. Our results show low expression levels of MHC class I molecules on the surface of these cells. An induction of MHC class I expression was observed when the cells were treated with IFN-gamma. Maximal induction of MHC class protein expression was observed at 48 h after treatment with concentrations above 5 ng/ml of IFN-gamma. Elevated MHC class I levels were sustained for 72 h after withdrawing IFN-gamma. Therefore, we introduced human cytomegalovirus (hCMV) US genes, which are known to be able to reduce MHC class I expression on the cell surface after infection, into M13SV1 cells. Cells transfected with the hCMV US2, US3, US6 or US11 genes exhibited a reduction (40-60%) of MHC class I expression compared with mock-transfected cells. These results suggest that human adult stem cells are capable of expressing high levels of MHC class I proteins, and thus may be rejected on transplantation unless they are modified. In addition, viral stealth mechanisms can be exploited for stem cell transplantation.
ISSN
1357-2725 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15381151

http://hdl.handle.net/10371/24576
DOI
https://doi.org/10.1016/j.biocel.2004.04.024
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College of Medicine/School of Medicine (의과대학/대학원)Anatomy (해부학전공)Journal Papers (저널논문_해부학전공)
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