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Long-term storage of Clonorchis sinensis metacercariae in vitro

Cited 8 time in Web of Science Cited 9 time in Scopus
Authors
Li, Shunyu; Kang, Hyoung Woo; Choi, Min-Ho; Hong, Sung-Tae
Issue Date
2006-06-13
Publisher
Springer International
Citation
Parasitol Res. 2006 Dec;100(1):25-9. Epub 2006 Jun 10.
Keywords
AnimalsCell SurvivalClonorchiasis/*epidemiologyClonorchis sinensis/cytology/drug effects/pathogenicity/*physiologyFishes/parasitologyFresh WaterHumansIncidenceIodoacetic Acid/pharmacologySurvivors
Abstract
Clonorchis sinensis metacercariae must often be stored for several months to meet laboratory requirements. In the present study, the authors evaluated morphological changes and infectivities of metacercariae kept in different storage solutions for 1, 3, and 6 months, i.e., PBS (phosphate buffered saline), PBS + antibiotics, PBS + iodoacetic acid (IAA), and PBS + antibiotics + IAA. Metacercariae stored in PBS containing antibiotics showed significantly higher survival rates and infectivities than those stored in PBS alone after 3 or 6 months. The metacercariae that had been stored for 6 months in solutions that were refreshed monthly had also significantly higher survival and infection rates than those stored in nonrefreshed solutions. Moreover, the addition of IAA, an inhibitor of cysteine proteinase, to these storage solutions reduced the survival and infectivity. The results of the present study confirm that the viabilities of C. sinensis metacercariae are best maintained in storage solutions containing antibiotics that are regularly refreshed.
ISSN
0932-0113 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16767424

http://hdl.handle.net/10371/27733
DOI
https://doi.org/10.1007/s00436-006-0242-8
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College of Medicine/School of Medicine (의과대학/대학원)Parasitology and Tropical Medicine (기생충학전공)Journal Papers (저널논문_기생충학전공)
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