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Characterization of two hepatitis B virus populations in a single Korean hepatocellular carcinoma patient with an HBeAg-negative serostatus: a novel X-Gene-deleted strain with inverted duplication sequences of upstream enhancer site II

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Authors
Kim, Hong; Jee, Youngmee; Mun, Ho-Suk; Park, Ju-Hee; Yoon, Jung-Hwan; Kim, Yoon-Jun; Lee, Hyo-Suk; Hyun, Jin-Won; Hwang, Eung-Soo; Cha, Chang-Yong; Kook, Yoon-Hoh; Kim, Bum-Joon
Issue Date
2007-06-16
Publisher
Karger
Citation
Intervirology. 2007;50(4):273-80. Epub 2007 Jun 15.
Keywords
Carcinoma, Hepatocellular/*virologyEnhancer Elements, Genetic/*geneticsEvolution, MolecularGene DeletionGene Duplication*Genome, ViralHepatitis B e Antigens/*bloodHepatitis B virus/*classification/genetics/isolation &purification/pathogenicityHumansLiver Neoplasms/*virologyMaleMiddle AgedMolecular Sequence DataRecombination, GeneticSequence Analysis, DNATrans-Activators/*genetics
Abstract
OBJECTIVES: The aim of the study was to elucidate mutation patterns related to hepatocarcinogenesis in a Korean hepatocellular carcinoma (HCC) patient. METHODS: We analyzed full genome sequences of 6 hepatitis B virus (HBV) clones from an HCC patient. RESULTS: This patient harbored 2 HBV populations with genomes of different lengths (3,221 and 2,212 bp). In addition, we found 2 characteristic features not described so far in the full-genome sequence of deleted strains. First, 3 large deletion events (847, 144 and 48 bp) and a premature termination of the 182th codon of the surface antigen could lead to truncated or possibly nonfunctional forms of all HBV proteins. Second, these showed a novel mutation type not reported to date, which is a complex of an inverted duplication of 36-bp sequences containing an upstream enhancer site II (UEII), a remote insertion, and a large deletion event of the X region by homologous recombination. CONCLUSION: The fact that UEII is a binding site of liver-specific nuclear factor, which is expressed only in highly differentiated liver cells such as cancerous HepG2, strongly suggests a relationship between this novel mutation and hepatocarcinogenesis in this patient.
ISSN
1423-0100 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17570929

http://hdl.handle.net/10371/28329
DOI
https://doi.org/10.1159/000103915
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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