Browse

Detection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAF600E mutation-prevalent area

DC Field Value Language
dc.contributor.authorChung, Ki-wook-
dc.contributor.authorYang, Sun Kyung-
dc.contributor.authorLee, Geon Kook-
dc.contributor.authorKim, Eun Young-
dc.contributor.authorKwon, Soim-
dc.contributor.authorLee, Sang Hyun-
dc.contributor.authorPark, Do Joon-
dc.contributor.authorLee, Hyun Sook-
dc.contributor.authorCho, Bo Youn-
dc.contributor.authorLee, Eun Sook-
dc.contributor.authorKim, Sun Wook-
dc.date.accessioned2010-01-07T06:38:20Z-
dc.date.available2010-01-07T06:38:20Z-
dc.date.issued2006-10-24-
dc.identifier.citationClin Endocrinol (Oxf). 2006 Nov;65(5):660-6.en
dc.identifier.issn0300-0664 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17054470-
dc.identifier.urihttp://hdl.handle.net/10371/28469-
dc.description.abstractBACKGROUND: Between 10 and 30% of the fine needle aspiration biopsies (FNABs) of thyroid nodules are diagnosed as 'indeterminate'. A molecular diagnostic method is needed to reduce unnecessary surgery in this group. In Korea, most thyroid cancer is the classic papillary type and the BRAF(V600E) mutation is highly prevalent. AIM: To evaluate the role of pre-operative detection of BRAF(V600E) mutation in the FNAB specimens of thyroid nodules in a BRAF(V600E) mutation-prevalent geographical area. PATIENTS AND METHODS: In 137 specimens of FNAB (107 papillary thyroid carcinomas (PTC); 3 follicular thyroid carcinomas (FTC); 2 undifferentiated thyroid carcinomas; 25 benign lesions), both direct DNA sequencing and PCR-RFLP were used for detecting the BRAF(V600E) mutation. The sensitivity and specificity were calculated. We analysed the association between BRAF(V600E) mutation and the clinico-pathological parameters. RESULTS: The BRAF(V600E) mutation was present in 93 (83%) of 112 thyroid cancers. Direct DNA sequencing showed a sensitivity of 83.0% and a specificity of 96.0%. The sensitivity and specificity of PCR-RFLP were 78.6% and 80.0%, respectively. Among 25 cases with indeterminate FNAB cytology, 8 patients had malignant lesions (5 PTC and 3 FTC). Three (60%) of 5 PTCs and 1 out of 17 benign lesions had BRAF(V600E) mutation (only one false positive case and the definitive pathology showed atypical nodular hyperplasia that could be a premalignant lesion). The diagnostic accuracy of this molecular method in only the 25 indeterminate nodules was 76% (19/25). No mutation was found in 3 FTCs. Among 107 PTCs, there was no significant association of the BRAF(V600E) mutation with the known risk factors. CONCLUSION: Detection of the BRAF(V600E) mutation in FNAB specimens refines the FNAB-cytology diagnosis, especially in a BRAF(V600E) mutation-prevalent area. Direct DNA sequencing was a more reliable method than PCR-RFLP for detecting the BRAF(V600E) mutation with a high sensitivity and specificity.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectAdenocarcinoma, Follicular/geneticsen
dc.subjectAdulten
dc.subjectAgeden
dc.subjectBiopsy, Fine-Needleen
dc.subjectCarcinoma, Papillary/ethnology/*genetics/pathologyen
dc.subjectCarcinoma, Papillary, Follicular/geneticsen
dc.subjectDNA Mutational Analysisen
dc.subjectDNA, Neoplasm/analysisen
dc.subjectDiagnosis, Differentialen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectKoreaen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subject*Point Mutationen
dc.subjectPolymorphism, Restriction Fragment Lengthen
dc.subjectProspective Studiesen
dc.subjectProto-Oncogene Proteins B-raf/*geneticsen
dc.subjectSensitivity and Specificityen
dc.subjectThyroid Neoplasms/ethnology/*genetics/pathologyen
dc.subjectThyroid Nodule/ethnology/*genetics/pathologyen
dc.titleDetection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAF600E mutation-prevalent areaen
dc.typeArticleen
dc.contributor.AlternativeAuthor정기욱-
dc.contributor.AlternativeAuthor양선경-
dc.contributor.AlternativeAuthor이건국-
dc.contributor.AlternativeAuthor김은영-
dc.contributor.AlternativeAuthor권소임-
dc.contributor.AlternativeAuthor이상현-
dc.contributor.AlternativeAuthor박도준-
dc.contributor.AlternativeAuthor이현숙-
dc.contributor.AlternativeAuthor조보연-
dc.contributor.AlternativeAuthor이은숙-
dc.contributor.AlternativeAuthor김선욱-
dc.identifier.doi10.1111/j.1365-2265.2006.02646.x-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse