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Detection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAF600E mutation-prevalent area
DC Field | Value | Language |
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dc.contributor.author | Chung, Ki-wook | - |
dc.contributor.author | Yang, Sun Kyung | - |
dc.contributor.author | Lee, Geon Kook | - |
dc.contributor.author | Kim, Eun Young | - |
dc.contributor.author | Kwon, Soim | - |
dc.contributor.author | Lee, Sang Hyun | - |
dc.contributor.author | Park, Do Joon | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.contributor.author | Cho, Bo Youn | - |
dc.contributor.author | Lee, Eun Sook | - |
dc.contributor.author | Kim, Sun Wook | - |
dc.date.accessioned | 2010-01-07T06:38:20Z | - |
dc.date.available | 2010-01-07T06:38:20Z | - |
dc.date.issued | 2006-10-24 | - |
dc.identifier.citation | Clin Endocrinol (Oxf). 2006 Nov;65(5):660-6. | en |
dc.identifier.issn | 0300-0664 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17054470 | - |
dc.identifier.uri | https://hdl.handle.net/10371/28469 | - |
dc.description.abstract | BACKGROUND: Between 10 and 30% of the fine needle aspiration biopsies (FNABs) of thyroid nodules are diagnosed as 'indeterminate'. A molecular diagnostic method is needed to reduce unnecessary surgery in this group. In Korea, most thyroid cancer is the classic papillary type and the BRAF(V600E) mutation is highly prevalent. AIM: To evaluate the role of pre-operative detection of BRAF(V600E) mutation in the FNAB specimens of thyroid nodules in a BRAF(V600E) mutation-prevalent geographical area. PATIENTS AND METHODS: In 137 specimens of FNAB (107 papillary thyroid carcinomas (PTC); 3 follicular thyroid carcinomas (FTC); 2 undifferentiated thyroid carcinomas; 25 benign lesions), both direct DNA sequencing and PCR-RFLP were used for detecting the BRAF(V600E) mutation. The sensitivity and specificity were calculated. We analysed the association between BRAF(V600E) mutation and the clinico-pathological parameters. RESULTS: The BRAF(V600E) mutation was present in 93 (83%) of 112 thyroid cancers. Direct DNA sequencing showed a sensitivity of 83.0% and a specificity of 96.0%. The sensitivity and specificity of PCR-RFLP were 78.6% and 80.0%, respectively. Among 25 cases with indeterminate FNAB cytology, 8 patients had malignant lesions (5 PTC and 3 FTC). Three (60%) of 5 PTCs and 1 out of 17 benign lesions had BRAF(V600E) mutation (only one false positive case and the definitive pathology showed atypical nodular hyperplasia that could be a premalignant lesion). The diagnostic accuracy of this molecular method in only the 25 indeterminate nodules was 76% (19/25). No mutation was found in 3 FTCs. Among 107 PTCs, there was no significant association of the BRAF(V600E) mutation with the known risk factors. CONCLUSION: Detection of the BRAF(V600E) mutation in FNAB specimens refines the FNAB-cytology diagnosis, especially in a BRAF(V600E) mutation-prevalent area. Direct DNA sequencing was a more reliable method than PCR-RFLP for detecting the BRAF(V600E) mutation with a high sensitivity and specificity. | en |
dc.language.iso | en | en |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Adenocarcinoma, Follicular/genetics | en |
dc.subject | Adult | en |
dc.subject | Aged | en |
dc.subject | Biopsy, Fine-Needle | en |
dc.subject | Carcinoma, Papillary/ethnology/*genetics/pathology | en |
dc.subject | Carcinoma, Papillary, Follicular/genetics | en |
dc.subject | DNA Mutational Analysis | en |
dc.subject | DNA, Neoplasm/analysis | en |
dc.subject | Diagnosis, Differential | en |
dc.subject | Female | en |
dc.subject | Humans | en |
dc.subject | Korea | en |
dc.subject | Male | en |
dc.subject | Middle Aged | en |
dc.subject | Polymorphism, Restriction Fragment Length | en |
dc.subject | Prospective Studies | en |
dc.subject | Proto-Oncogene Proteins B-raf/*genetics | en |
dc.subject | Sensitivity and Specificity | en |
dc.subject | Thyroid Neoplasms/ethnology/*genetics/pathology | en |
dc.subject | Thyroid Nodule/ethnology/*genetics/pathology | en |
dc.subject | Point Mutation | - |
dc.title | Detection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAF600E mutation-prevalent area | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 정기욱 | - |
dc.contributor.AlternativeAuthor | 양선경 | - |
dc.contributor.AlternativeAuthor | 이건국 | - |
dc.contributor.AlternativeAuthor | 김은영 | - |
dc.contributor.AlternativeAuthor | 권소임 | - |
dc.contributor.AlternativeAuthor | 이상현 | - |
dc.contributor.AlternativeAuthor | 박도준 | - |
dc.contributor.AlternativeAuthor | 이현숙 | - |
dc.contributor.AlternativeAuthor | 조보연 | - |
dc.contributor.AlternativeAuthor | 이은숙 | - |
dc.contributor.AlternativeAuthor | 김선욱 | - |
dc.identifier.doi | 10.1111/j.1365-2265.2006.02646.x | - |
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