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The identification of a Clonorchis sinensis gene encoding an antigenic egg protein

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Authors
Lee, Mejeong; Chung, Young-Bae; Lee, Suk-Keun; Chung, Byung-Suk; Li, Shunyu; Choi, Min-Ho; Hong, Sung-Tae
Issue Date
2004-12-24
Publisher
Springer International
Citation
Parasitol Res. 2005 Feb;95(3):224-6. Epub 2004 Dec 23.
Keywords
Amino Acid SequenceAnimalsAntibodies, Helminth/immunologyAntigens, Helminth/chemistry/*genetics/*immunologyCloning, MolecularClonorchiasis/diagnosis/parasitologyClonorchis sinensis/genetics/*growth & development/*immunology/isolation &purificationEgg Proteins/chemistry/genetics/immunologyHelminth Proteins/chemistry/*genetics/immunologyHumansImmune Sera/*immunologyImmunohistochemistryMiceMice, Inbred BALB CMolecular Sequence DataRecombinant Proteins/immunologySequence Analysis, DNA
Abstract
The cDNA library of Clonorchis sinensis was screened for genes encoding antigenic proteins by using sera from clonorchiasis patients. A gene of 888 bp encoding a 28-kDa protein (Cs28) was cloned and found to contain a high percentage of glycine (20%), tyrosine (11%), and lysine (11%). The amino acid sequence of Cs28 showed 60% homology with the vitelline B precursor protein of Opisthorchis viverrini and of 33% homology with the vitelline B1 and B2 proteins of Fasciola hepatica. A strong positive reaction was observed in the intrauterine eggs of adult C. sinensis by immunohistochemical analysis using specific immune sera against recombinant Cs28 protein (rCs28). By immunoblot analysis, rCs28 displayed an antigenic reaction with 73% of the serum samples from 115 cases of clonorchiasis. In addition, it cross-reacted with the sera of 77.5% of 40 opisthorchiasis cases, 90% of 20 schistosomiasis cases, and 50% of 10 paragonimiasis cases. However, no cross-reactions were observed with the sera of sparganosis or cysticercosis patients. In conclusion, the Cs28 protein was identified as an egg protein of C. sinensis and as an antigen common to the trematode species examined.
ISSN
0932-0113 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15616856

http://hdl.handle.net/10371/28484
DOI
https://doi.org/10.1007/s00436-004-1277-3
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College of Medicine/School of Medicine (의과대학/대학원)Parasitology and Tropical Medicine (기생충학전공)Journal Papers (저널논문_기생충학전공)
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