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Arsenic trioxide represses constitutive activation of NF-kappaB and COX-2 expression in human acute myeloid leukemia, HL-60

Cited 42 time in Web of Science Cited 45 time in Scopus
Authors
Han, Seong-Su; Kim, Kihyun; Hahm, Eun-Ryeong; Park, Chan H; Kimler, Bruce F; Lee, Sook J; Lee, Se-Hoon; Kim, Won S; Jung, Chul Won; Park, Keunchil; Kim, Jingook; Yoon, Sung-Soo; Lee, Je-Ho; Park, Seyeon
Issue Date
2004-11-18
Publisher
Wiley-Blackwell
Citation
J Cell Biochem. 2005 Mar 1;94(4):695-707.
Keywords
Apoptosis/drug effectsArsenicals/*pharmacologyCyclooxygenase 2DNA/metabolismEnzyme Activation/drug effectsGene Expression Regulation, Enzymologic/drug effectsGene Expression Regulation, Neoplastic/*drug effectsGlutathione/metabolismHL-60 CellsHumansHydrogen Peroxide/metabolismI-kappa B KinaseLeukemia, Myeloid, Acute/enzymology/genetics/metabolismMembrane ProteinsNF-kappa B/chemistry/*metabolismOxides/*pharmacologyProstaglandin-Endoperoxide Synthases/*metabolismProtein Binding/drug effectsProtein Subunits/chemistry/metabolismProtein Transport/drug effectsProtein-Serine-Threonine Kinases/metabolism
Abstract
It has been proposed that eukaryotic nuclear factor nuclear factor kappa-B (NF-kappaB) and cyclooxygenase-2 (COX-2) are implicated in the pathogenesis of many human diseases including cancer. Arsenic has been widely used in medicine in Oriental countries. Recent studies have shown that arsenic trioxide (As(2)O(3)) could induce in vitro growth inhibition and apoptosis of malignant lymphocytes, and myeloma cells. However, the molecular mechanisms by which As(2)O(3) initiates cellular signaling toward cell death are still unclear. In the present study, the effects of As(2)O(3) on NF-kappaB and COX-2 expression in HL-60 cells were investigated. As(2)O(3) suppressed DNA-binding activity of NF-kappaB composed of p65/p50 heterodimer through preventing the degradation of IkappaB-alpha and the nuclear translocation of p65 subsequently as well as interrupting the binding of NF-kappaB with their consensus sequences. Inhibitory effect of As(2)O(3) on NF-kappaB DNA activity was dependent upon intracellular glutathione (GSH) and H(2)O(2) level, but not superoxide anion. Futhermore, we found that As(2)O(3) also downregulated the expression of COX-2, which has NF-kappaB binding site on its promoter through repressing the NF-kappaB DNA-binding activity.
ISSN
0730-2312 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15547942

http://hdl.handle.net/10371/29067
DOI
https://doi.org/10.1002/jcb.20337
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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