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Magnetic resonance spectroscopy for measuring the biodistribution and in situ in vivo pharmacokinetics of fluorinated compounds: validation using an investigation of liver and heart disposition of tecastemizole

Cited 9 time in Web of Science Cited 10 time in Scopus
Authors
Schneider, E; Bolo, N R; Frederick, B; Wilkinson, S; Hirashima, F; Nassar, L; Lyoo, I K; Koch, P; Jones, S; Hwang, J; Sung, Y; Villafuerte, R A; Maier, G; Hsu, R; Hashoian, R; Renshaw, P F
Issue Date
2006-06-23
Publisher
Wiley-Blackwell
Citation
J Clin Pharm Ther. 2006 Jun;31(3):261-73.
Keywords
AdultAnimalsAnti-Allergic Agents/*pharmacokineticsBenzimidazoles/*pharmacokineticsChromatography, High Pressure LiquidDogsDose-Response Relationship, DrugEcho-Planar ImagingFemaleFluorine Compounds/*pharmacokineticsFluorine Radioisotopes/diagnostic useHalf-LifeHumansLiver/*metabolismMagnetic Resonance SpectroscopyMaleMiddle AgedMyocardium/*metabolismPiperidines/*pharmacokineticsReproducibility of ResultsTissue Distribution
Abstract
BACKGROUND AND OBJECTIVE: The study of biodistribution and in situ pharmacokinetics is a challenging, but sometimes very important, aspect of premarketing characterization of drugs. We aimed to develop a non-invasive fluorine magnetic resonance (MR) spectroscopic method for the absolute quantitation of a mono-fluorinated compound and of its metabolites in the heart and liver of healthy subjects for this purpose. METHOD: We used fluorine MR spectroscopy (MRS) at 4 T (Tesla) and external standardization in an open label multiple-dose study. Twenty-three healthy adult subjects were enrolled in the study. The surface coil localized fluorine MR spectrum was monitored in the heart and liver at baseline and after oral administration of multiple doses of tecastemizole. Steady-state measurements were made at set time points that depended upon dose, and washout measurements were made only on subjects in which in vivo fluorine signal was observed. RESULTS AND DISCUSSION: At 4 T, under the given experimental conditions, the method had a lower limit of quantitation (LLOQ) of about 2.6 microm and a limit of detection (LOD) of about 0.3 microm for solution state samples (linewidth approximately 15 Hz). The measurement reproducibility was 6.4% using a 50 microm phantom. The effect of MR operator and spectral analyst on the calculated calibration curve slope was small, with inter-rater correlation coefficients of 0.999 and 0.998 respectively. MR signal from fluorine-containing tecastemizole-related moieties was observed in situ only at day 8 in the liver of three of five subjects dosed at 270 mg/day. The average in situ concentration was estimated to be 58+/-22 microm, with an average test-retest reproducibility of 216%. Extrapolating the in vitro results to human measurements, with an approximate linewidth of 250 Hz, predicts in situ LOD and LLOQ values of approximately 6 and 44 microm respectively. However, the human study had a fluorine MRS LOD of approximately 20 microm. The decrease in sensitivity and the increase in variability of the in vivo, in situ measurements compared with the validation study most likely arose from coil placement and incomplete rephasing of the MR signal by the respiratory phase compensation method. CONCLUSION: The measured concentrations were the lowest ever recorded for a multi-dose exogenous mono-fluorinated compound in the human liver using a validated fluorine MR quantitation method. The proposed non-invasive MR method for studying the biodistribution and in situ pharmacokinetics of mono-fluorinated compounds in the liver and heart should have broader application to the development of non-invasive biomarkers.
ISSN
0269-4727 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16789992

http://hdl.handle.net/10371/29257
DOI
https://doi.org/10.1111/j.1365-2710.2006.00735.x
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College of Medicine/School of Medicine (의과대학/대학원)Psychiatry (정신과학전공)Journal Papers (저널논문_정신과학전공)
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