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Cabin1 represses MEF2 transcriptional activity by association with a methyltransferase, SUV39H1
DC Field | Value | Language |
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dc.contributor.author | Jang, Hyonchol | - |
dc.contributor.author | Choi, Doo-Eun | - |
dc.contributor.author | Kim, Hyungsoo | - |
dc.contributor.author | Cho, Eun-Jung | - |
dc.contributor.author | Youn, Hong-Duk | - |
dc.date.accessioned | 2010-01-12T04:34:17Z | - |
dc.date.available | 2010-01-12T04:34:17Z | - |
dc.date.issued | 2006-12-19 | - |
dc.identifier.citation | J Biol Chem. 2007 Apr 13;282(15):11172-9. Epub 2006 Dec 17. | en |
dc.identifier.issn | 0021-9258 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17172641 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29651 | - |
dc.description.abstract | Myocyte enhancer factor 2 (MEF2) plays pivotal roles in various biological processes, and its transcriptional activity is regulated by histone acetylation/deacetylation enzymes in a calcium-dependent fashion. A calcineurin-binding protein 1 (Cabin1) has been shown to participate in repression of MEF2 by recruiting mSin3 and its associated histone deacetylases. Here, we report that histone methylation also takes a part in Cabin1-mediated repression of MEF2. Immunoprecipitate of Cabin1 complex can methylate histone H3 by association with SUV39H1. SUV39H1 increased Cabin1-mediated repression of MEF2 transcriptional activity in MEF2-targeting promoters. The SUV39H1 was revealed to bind to the 501-900-amino acid region of Cabin1, which was distinct from its histone deacetylase-recruiting domain. In addition, the Gal4-Cabin1-(501-900) alone repressed a constitutively active Gal4-tk-promoter, indicating that Cabin1 recruits SUV39H1 and represses transcriptional activity. Finally, both SUV39H1 and Cabin1 were shown to bind on the MEF2 target promoter in a calcium-dependent manner. Thus, Cabin1 recruits chromatin-modifying enzymes, both histone deacetylases and a histone methyltransferase, to repress MEF2 transcriptional activity. | en |
dc.language.iso | en | en |
dc.publisher | American Society for Biochemistry and Molecular Biology | en |
dc.subject | Amino Acids/genetics/metabolism | en |
dc.subject | Calcium/metabolism | en |
dc.subject | Cell Line | en |
dc.subject | Histone-Lysine N-Methyltransferase/genetics/*metabolism | en |
dc.subject | Humans | en |
dc.subject | Myogenic Regulatory Factors/*genetics/*metabolism | en |
dc.subject | Phosphoproteins/genetics/*metabolism | en |
dc.subject | Promoter Regions, Genetic/genetics | en |
dc.subject | Protein Binding | en |
dc.subject | Protein Methyltransferases | en |
dc.subject | Transcription, Genetic/*genetics | en |
dc.title | Cabin1 represses MEF2 transcriptional activity by association with a methyltransferase, SUV39H1 | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 장현철 | - |
dc.contributor.AlternativeAuthor | 김형수 | - |
dc.contributor.AlternativeAuthor | 조은정 | - |
dc.contributor.AlternativeAuthor | 윤홍덕 | - |
dc.identifier.doi | 10.1074/jbc.M611199200 | - |
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