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Intercellular adhesion molecule-1 is upregulated in ischemic muscle, which mediates trafficking of endothelial progenitor cells
Cited 80 time in
Web of Science
Cited 83 time in Scopus
- Authors
- Issue Date
- 2006-02-25
- Publisher
- American Heart Association
- Citation
- Arterioscler Thromb Vasc Biol. 2006 May;26(5):1066-72. Epub 2006 Feb 23.
- Keywords
- Animals ; Antigens, CD18/genetics ; Antigens, CD31/physiology ; Cell Adhesion ; Cell Movement ; Endothelial Cells/*physiology ; Hematopoietic Stem Cells/*physiology ; Hindlimb/blood supply ; Inflammation/etiology ; Intercellular Adhesion Molecule-1/*physiology ; Ischemia/*metabolism ; Mice ; Mice, Inbred C57BL ; Muscle, Skeletal/*blood supply ; Neovascularization, Physiologic ; Stem Cell Transplantation
- Abstract
- BACKGROUND: Trafficking of transplanted endothelial progenitor cells (EPCs) to an ischemic organ is a critical step in neovascularization. This study was performed to elucidate the molecular mechanism of EPC trafficking in terms of adhesion molecules. METHODS AND RESULTS: Using murine hindlimb ischemia model, we examined expressions of E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and platelet-endothelial cell adhesion molecule-1 (PECAM-1) in ischemic muscle by immunofluorescence. ICAM-1 was overexpressed in ischemic muscle compared with nonischemic muscle, whereas expressions of E-selectin, VCAM-1, and PECAM-1 did not show that much difference. ICAM-1 was also upregulated by hypoxia in murine endothelial cells (ECs) as assessed by immunoblot and flow cytometry. EPCs were attached to ECs specifically through ICAM-1/beta-2 integrin interaction in vitro. When EPCs were labeled with fluorescent dye or radioisotope (Tc-99m-HMPAO) and systemically administrated in vivo, EPCs preferentially homed to ischemic muscle. By blocking ICAM-1, EPCs entrapment to ischemic limb in vivo was significantly reduced and neovascularization induced by EPC transplantation was attenuated. CONCLUSIONS: ICAM-1 is upregulated by ischemia, and this is closely associated with EPCs entrapment to ischemic limb. Our findings suggest that ICAM-1 expression might be important in regulating the process of neovascularization through its ability to recruit EPCs.
- ISSN
- 1524-4636 (Electronic)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16497992
https://hdl.handle.net/10371/44047
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