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Overexpression of USF increases TGF-beta1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Keun-Sook | - |
dc.contributor.author | Jung, Hye Seung | - |
dc.contributor.author | Chung, Yun Jae | - |
dc.contributor.author | Jung, Tae Sik | - |
dc.contributor.author | Jang, Hye Won | - |
dc.contributor.author | Lee, Myung-Shik | - |
dc.contributor.author | Kim, Kwang-Won | - |
dc.contributor.author | Chung, Jae Hoon | - |
dc.date.accessioned | 2010-01-28T08:28:11Z | - |
dc.date.available | 2010-01-28T08:28:11Z | - |
dc.date.issued | 2008-10-29 | - |
dc.identifier.citation | J Korean Med Sci. 2008 Oct;23(5):870-6. | en |
dc.identifier.issn | 1011-8934 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18955796 | - |
dc.identifier.uri | http://synapse.koreamed.org/Synapse/Data/PDFData/0063JKMS/jkms-23-870.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/46310 | - |
dc.description.abstract | Transforming growth factor-beta1 (TGF-beta1) is a potent inhibitor of cellular growth and proliferation by G1 phase arrest or apoptosis. We investigated the association of TGF-beta1 with the anti-proliferative effect of upstream stimulatory factor (USF) in Fischer rat thyroid cell line (FRTL-5) cells. [methyl-(3)H] thymidine uptake was measured after treatment of FRTL-5 cells with TGF-beta1 to identify its anti-proliferative effect. USF-1 and USF-2 proteins were in vitro translated, and an electrophoretic mobility shift assay was performed to identify the interaction between USF and the TGF-beta1 promoter. FRTL-5 cells were transfected with USF cDNA, and then the expression of TGF-beta1 was examined with Northern and Western blotting. The cell cycle-regulating proteins associated with TGF-beta1 were also measured. TGF-beta1 significantly inhibited [methyl-(3)H] thymidine uptake in FRTL-5 cells. Two specific binding sites for USF were found in the TGF-beta1 promoter: -1,846 approximately -1,841 (CACATG) and -621 approximately -616 (CATGTG). Overexpression of USF increased both the mRNA levels and protein levels of TGF-beta1. However, the expression of cyclin D1, CDK4, cyclin E, and CDK2, and the phosphorylation of retinoblastoma protein remained unchanged. Overexpression of USF in FRTL-5 cells increased the expression of TGF-beta10 through specific binding to TGF-beta1 promoter. However, the USF-induced expression of TGF-beta1 did not cause G1 arrest. | en |
dc.language.iso | en | en |
dc.publisher | Korean Academy of Medical Science | en |
dc.subject | Animals | en |
dc.subject | Binding Sites | en |
dc.subject | Cell Cycle | en |
dc.subject | Cell Line | en |
dc.subject | G1 Phase | en |
dc.subject | Promoter Regions, Genetic | en |
dc.subject | Protein Biosynthesis | en |
dc.subject | Rats | en |
dc.subject | Thymidine/chemistry | en |
dc.subject | Transfection | en |
dc.subject | Transforming Growth Factor beta1/metabolism | en |
dc.subject | Upstream Stimulatory Factors/*metabolism | en |
dc.subject | Apoptosis | - |
dc.subject | Gene Expression Regulation | - |
dc.title | Overexpression of USF increases TGF-beta1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김근숙 | - |
dc.contributor.AlternativeAuthor | 정혜승 | - |
dc.contributor.AlternativeAuthor | 정윤재 | - |
dc.contributor.AlternativeAuthor | 정태식 | - |
dc.contributor.AlternativeAuthor | 장혜원 | - |
dc.contributor.AlternativeAuthor | 이명식 | - |
dc.contributor.AlternativeAuthor | 김광원 | - |
dc.contributor.AlternativeAuthor | 정재훈 | - |
dc.identifier.doi | 10.3346/jkms.2008.23.5.870 | - |
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