Browse

Differential effects of secreted frizzled-related proteins (sFRPs) on osteoblastic differentiation of mouse mesenchymal cells and apoptosis of osteoblasts

Cited 32 time in Web of Science Cited 31 time in Scopus
Authors
Cho, Sun Wook; Her, Sun Ju; Sun, Hyun Jin; Choi, Ok Kyong; Yang, Jae-Yeon; Kim, Sang Wan; Kim, Seong Yeon; Shin, Chan Soo
Issue Date
2008-01-02
Publisher
Elsevier
Citation
Biochem Biophys Res Commun. 2008 Mar 7;367(2):399-405. Epub 2007 Dec 31.
Keywords
AnimalsApoptosis/physiologyCell Differentiation/physiologyCell LineGlycoproteins/*metabolismMembrane Proteins/*metabolismMesenchymal Stem Cells/*cytology/*physiologyMiceMice, Inbred C3HOsteoblasts/*cytology/*physiologyProto-Oncogene Proteins/*metabolism
Abstract
Secreted frizzled-related proteins (sFRPs) are modulators of Wnt signaling. This study was undertaken for definitive assessment of contribution of different sFRPs in osteoblastic differentiation of mesenchymal progenitor cells and apoptosis of osteoblasts. Treatment of C3H10T1/2 cells with sFRP-2 at concentrations of 10, 50, and 100nM and sFRP-4 at low concentrations (5nM) significantly increased Wnt-3A-induced alkaline phosphatase (ALP) activities, whereas sFRP-1 or 3 did not. Retroviral transduction of the sFRP-2 but not other sFRPs also significantly enhanced ALP activity induced by beta-glycerophosphate and ascorbic acid. Furthermore, transfection of all the sFRP expression vectors significantly increased beta-catenin/TCF reporter activity and the effects were most prominent with sFRP-2 and -4. In osteoblast apoptosis assay, only sFRP-3 increased etoposide-induced apoptosis in MC3T3-E1 mouse osteoblasts. In conclusion, we found that different repertoires of sFRPs exert differential effects on osteoblastic differentiation of mouse mesenchymal cells and cellular apoptosis of mouse osteoblasts in vitro.
ISSN
1090-2104 (Electronic)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4RGFR6X-8&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=0cbed86cf7ebb081c9f33555bb0252e5

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18166153

http://hdl.handle.net/10371/46595
DOI
https://doi.org/10.1016/j.bbrc.2007.12.128
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse