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방사선의 선량변화가 수종의 정상세포와 종양세포주의 세포활성도와 apoptosis 유발에 미치는 영향 : Effect of Radiation Dosage Changes on the Cell Viability and the Apoptosis Induction on Normal and Tumorigenic Cells
DC Field | Value | Language |
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dc.contributor.author | 박인우 | - |
dc.contributor.author | 이삼선 | - |
dc.contributor.author | 허민석 | - |
dc.contributor.author | 최순철 | - |
dc.date.accessioned | 2010-02-01T09:41:01Z | - |
dc.date.available | 2010-02-01T09:41:01Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | 치과방사선 1999;29: 435-449 | en |
dc.identifier.issn | 1225-049X | - |
dc.identifier.uri | https://hdl.handle.net/10371/47588 | - |
dc.description.abstract | Purpose : The study was aimed to detect the differences in the cell viability and the apoptosis induction after irradiation on normal and tumorigenic cells. Materials and Methods : The study. that was generated for two human normal cells(RHEK, HGF-l) and two human tumor cells(KB. HT-1080). was tested using MTT assay at 1 day and 3 day after irradiation and TUNEL assay under confocal laser scanning microscope at 1 day after irradiation. Single irradiation of 0.5. 1, 2. 4. and 8Gy were applied to the cells. The two fractions of 1. 2. 4. and 8Gy were separated with a 4-hour time interval. The irradiation was done with 5.38Gy/min dose rate using Cs-137 irradiator at room temperature. Results and Conclusions : 1. In 3-day group. the cell viability of HGF-1 cell was significantly decreased at 2. 4 and 8Gy irradiation, the cell viability of KB cell was significantly decreased at 8Gy irradiation and the cell viability of HT-I080 cell was significantly decreased at 4 and 8Gy irradiation. 2. There was significant difference between RHEK and KB cell line in the cell viability of 3-day group at 8Gy irradiation. There was significant difference between RHEK and HGF-1 cell line in the cell viability of 3-day group at 4 and 8Gy irradiation. 3. There was a significantly decreased cell viability in 3-day group than those in 1-day group at 2. 4 and 8Gy on HGF-1 cell. at 4 and 8Gy on HT-I080 cell. at 8Gy on KB cell. 4. We could detect DNA fragmented cells only on KB cell. Number of apoptotic cells of KB cell was significantly increased at 4 and 8Gy irradiation. However, there was no correlation between cell viability and apoptosis. 5. On all 4 cell lines, there were no differences between single and split irradiation method in cell viability and apoptosis. | en |
dc.language.iso | ko | en |
dc.publisher | 대한구강악안면방사선학회 | en |
dc.subject | irradiation | en |
dc.subject | cell viability | en |
dc.subject | apoptosis | en |
dc.subject | MTT assay | en |
dc.subject | TUNEL assay | en |
dc.title | 방사선의 선량변화가 수종의 정상세포와 종양세포주의 세포활성도와 apoptosis 유발에 미치는 영향 | en |
dc.title.alternative | Effect of Radiation Dosage Changes on the Cell Viability and the Apoptosis Induction on Normal and Tumorigenic Cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | Park, In-Woo | - |
dc.contributor.AlternativeAuthor | Lee, Sam-Sun | - |
dc.contributor.AlternativeAuthor | Heo, Min-Suk | - |
dc.contributor.AlternativeAuthor | Choi, Soon-Chul | - |
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