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말초신경 재건을 위한 인회석 박막 코팅 미세공성 신경재생관(nerve conduit)의 개발

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Authors
이종호; 황순정; 최원재; 김성민; 김남열; 이은진; 안강민; 명훈; 서병무; 최진영; 정필훈; 김명진; 김현만; 박경표; 김중수
Issue Date
2003
Publisher
대한구강악안면외과학회
Citation
대한구강악안면외과학회지 29(3):151-156.
Keywords
Nerve regenerationMilipore conduitsSchwann cellsCalcium phosphate coating
Abstract
This study was performed to develop a useful nerve conduit which provides favorable environment for Schwann cell viability and proliferation. Milipore membrane of 0.45μm pore size was selected because it permits nutritional inflow from the outside of the conduit and prevents from invading the fibrotic tissue into the conduit. The membrane was rolled and sealed to form a conduit of 2mm diameter and 20mm length. To improve the axonal regeneration and to render better environment for endogenous and exogenous Schwann
cell behaviour, the microgeometry and surface of conduit was modified by coating with thin film of calcium phosphate.
Cellular viability within the conduit and attachment to its wall were assessed with MTT assay and SEM study. Milipore filter conduit showed significantly higher rate of Schwann cell attachment and viability than the culture dish. However, the reverse was true in case of fibroblast. Coating with thin film of low crystalline calcium phosphate made more favorable environment for both cells with minimal change of pore size.
These findings means the porous calcium phosphate coated milipore nerve conduit can provide much favorable environment for endogenous Schwann cell proliferation and exogenous ones, which are filled within the conduit for the more advanced strategy of peripheral nerve regeneration, with potential of reducing fibrotic tissue production.
ISSN
1225-1585
Language
Korean
URI
http://uci.or.kr/ G300-j12251585.v29n03p151

http://hdl.handle.net/10371/47715
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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