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E.coli Lipopolysaccharides로 유도된 사람 호중구중에서 CD14, Toll-Like receptors, cytoskeletal inhibitors 그리고 NF-kB inhibitor가 MMP-8 분비에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 양승민 | - |
dc.contributor.author | 김태일 | - |
dc.contributor.author | 설양조 | - |
dc.contributor.author | 이용무 | - |
dc.contributor.author | 구영 | - |
dc.contributor.author | 정종평 | - |
dc.contributor.author | 한수부 | - |
dc.contributor.author | 류인철 | - |
dc.date.accessioned | 2010-02-01T09:53:59Z | - |
dc.date.available | 2010-02-01T09:53:59Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | 대한치주과학회지 2005;35(2):427-436. | en |
dc.identifier.issn | 0250-3352 | - |
dc.identifier.uri | http://uci.or.kr/G100:I100-KOI(KISTI1.1003/JNL.JAKO200521452724037) | - |
dc.identifier.uri | https://hdl.handle.net/10371/47850 | - |
dc.description.abstract | Objective: MMP-8 is a neutrophil enzyme and its level increases in some inflammatory diseases, including periodontal disease. We knew that the lipopolysaccharide of E.coli(E-LPS) induced MMP-8 release from human neutrophils. E-LPS is known to induce the production and release of inflammatory cytokines through CD14, Toll-like receptor(TLR). In the present study, we investigated whether MMP-8 release by E-LPS is induced via CD14-TLR pathway and the cellular mechanism of MMP-8 release in human neutrophils. Material and methods: Human neutrophils were isolated from the peripheral blood of healthy donors and pre-incubated in medium containing antibodies against CD14, anti-TLR2 and anti-TLR4 or several inhibitors of microtubules and microfilaments and then incubated with E-LPS. The cells were treated TPCK and E-LPS simultaneously. The MMP-8amount in the culture medium was determined using ELISA. Results: E-LPS increased MMP-8release from neutrophils and its induction was inhibited by anti-CD14 and anti-TLR4 but not by anti-TLR2 antibodies. The inhibitors of microtubule and microfilament polymerization significantly decreased E-LPS-induced MMP-8release. TPCK inhibited E-LPS-induced MMP-8 release. Conclusion: These results suggest that MMP-8 release is induced by E-LPS via the CD14-TLR4 signal pathway in human neutrophils and may be depedent on microtubule and microfilament systems and pathway. | en |
dc.language.iso | ko | en |
dc.publisher | 대한치주과학회 | en |
dc.subject | Human Neutrophil | en |
dc.subject | CD14 | en |
dc.subject | TLRs | en |
dc.subject | Cytoskeletal Inhibitors | en |
dc.subject | NF-B inhibitor | en |
dc.subject | MMP-8 | en |
dc.title | E.coli Lipopolysaccharides로 유도된 사람 호중구중에서 CD14, Toll-Like receptors, cytoskeletal inhibitors 그리고 NF-kB inhibitor가 MMP-8 분비에 미치는 영향 | en |
dc.type | Article | en |
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