The Proton Nuclear Magnetic Resonance Spectral Analysis of Human Blood Plasma Lipoprotein

Abstract

300 MHZ proton NMR spectra of human blood plasma were analyzed by deconvolution of spectrum, and we compared its results with Fossel's test in normal (15 cases), liver cancer (14 cases), and other cncer (14 cases) groups. This analysis had enabled us to obtain dynamic characteristics of each individual lipoprotein. As a result of deconvolution method, the VLDL and chylomicron intensity level were found to be elevated in the patients with liver cancer. Moment ratio values of CH2 resonance in the raw spectrum were found to be higher than the normal group for patients with malignant tumors other than liver cancer. These differences between the three groups could not be found in the conventional Fossel's test. We could simulate plasma spectra by addition of spectra of individual liporoteins through deconvolution method. Further clinical trials in larger populations and additional biochemical method may shed new light on many of clinical and biochemical interests for knowing charateristics about lipoprotein not separated from blood and the background of Fossel test.