S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 30 No.3 (1989)
In Vitro Cultivation and Transplantation of Mouse Embryos: Comparative Analysis of Effects of Bovine Serum Albumin and Sodium Lactate
생쥐 수정란의 시험관내 배양 및 이식 : Bovine serum albumin 및 sodium lactate 효과의 비교분석
- Baik, Sang Ho; Jeong, Goo Bo; Hwang, Douk Ho
- Issue Date
- Seoul J Med, Vol.30 No.3, pp. 143-148
- Two-cell embryos were recovered from ICR, Balb/c, and Fl hybrid (CBA X
C57Bl/6) females, and cultured in vitro in the Ham's F-I0 medium supplemented with 0.1
% BSA, 20 mM Na-lactate, or 10 mM HEPES. With Fl hybrid embryos, there were significantly
higher development in Ham's F-I0 medium supplemented with 0.1% BSA (79.1%)
and lower development in that supplemented with 10 mM HEPES. Significant differences
between strains were seen also, and preimplantation deveiopment was significantly better
with the embryos of the Flhybrid strain and was lower with the embryos of the Balb/c strain.
It was concluded that BSA and Na-lactate are the important factors affecting preimplantation
development, and embryos of the Fl hybrid strain are less sensitive to culture conditions.
After transplantation of blastocysts cultured in vitro, the pregnancy rate of recipient mice
and implantation rate of transferred embryos were 60% and 32%, respectively.