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Only Tetracaine, among local anesthetics, induces apoptosis by generation of reactive oxygen species in rat cortical astrocytes

Cited 13 time in Web of Science Cited 14 time in Scopus
Authors
Kim, H. J; Lee, W. Y; Park, C. J; Shin, T. J; Yum, K. W; Seo, K. S
Issue Date
2009-08
Publisher
Oxford University Press
Citation
BRITISH JOURNAL OF ANAESTHESIA 2009; 103: 719-725
Keywords
anaesthetics localtetracainecomplicationsneurologicaltoxicitylocal anaesthetics
Abstract
Background: The potential risks of neurotoxicity due to local anaesthetics after regional anaesthesia have been suggested recently. To evaluate the neurotoxicity of commonly used local anaesthetics, primary cultured rat cortical astrocytes were treated with lidocaine, ropivacaine, bupivacaine, levobupivacaine, and tetracaine.

Methods: Cell death after local anaesthetic treatment was evaluated with a lactate dehydrogenase (LDH) assay. To examine the mechanisms of cell death, reactive oxygen species (ROS) measurement and western blots of poly-ADP ribose polymerase (PARP), procaspase-3, and mitogen-activated protein kinases family members were performed.

Results: Of the local anaesthetics, which were applied at <1 mM for 18 h, only tetracaine significantly increased LDH leakage (P<0.05) and cell death in a dose- and time-dependent manner. Hoechst 33258–propidium iodide staining and western blots with PARP and procaspase-3 antibodies suggested that tetracaine induced apoptosis. ROS levels increased 2-fold at 30 min after tetracaine treatment compared with the control and then decreased. The antioxidants, N-acetylcysteine and trolox, markedly inhibited tetracaine-induced apoptosis.

Conclusions: Tetracaine induced apoptosis through ROS generation. Further studies focusing on the neurotoxicity of tetracaine are needed.
ISSN
0007-0912
Language
English
URI
http://hdl.handle.net/10371/60095
DOI
https://doi.org/10.1093/bja/aep237
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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