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Patterning and separating infected bacteria using host-parasite and virus-antibody interactions

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Authors
Suh, Kahp Y.; Khademhosseini, Ali; Yoo, Pil J.; Langer, Robert
Issue Date
2004-09
Publisher
Springer Verlag
Citation
Biomedical Microdevices, 6, 223-229 (2004)
Keywords
poly(ethylene glycol) microstructureshost-parasite interactionscapillary lithographybacteriapatterningSELF-ASSEMBLED MONOLAYERSPOLY-L-LYSINEESCHERICHIA-COLISURFACESADHESIONCELLMICROSTRUCTURESPROTEIN
Abstract
Bacteria were selectively deposited on substrates patterned with poly(ethylene glycol) (PEG) microstructures by using host-parasite and virus-antibody interactions. In this scheme viruses were used to attach onto a host bacterium, Escherichia coli (E. coli). The E. coli expressing the virus were selectively adhered to the regions pretreated with an antibody against the virus proteins while E. coli without the virus showed no selectivity. Single or aggregated cell arrays were fabricated depending on the initial pattern size with respect to the size of E. coli. The current approach could be a general route to spatially positioning or controlling adhesion of other biological species that are not accessible by conventional methods and as a tool for separating and isolating specific cell populations based on host-parasite interactions.
ISSN
1387-2176
Language
English
URI
http://hdl.handle.net/10371/6218
DOI
https://doi.org/10.1023/B:BMMD.0000042052.47444.9a
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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