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Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures

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Authors
Khademhosseini, Ali; Suh, Kahp Y.; Yang, Jen M.; Eng, George; Yeh, Judy; Levenberg, Shulamit; Langer, Robert
Issue Date
2004-08
Publisher
Elsevier
Citation
Biomaterials 2004;25:3583-92
Keywords
patterned co-culturesLayer-by-layerHyaluronic acidpoly-L-lysineEMBRYONIC STEM-CELLSDRUG-DELIVERYHEPATOCYTESSURFACESSYSTEMSPROTEINADHESIONDEXTRANCULTUREFILMS
Abstract
Anovel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Nonbiofouling
hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic
adsorption of poly-l-lysine (PLL) to HApatterns was used to switch the HAsurfaces from cell repulsive to adherent thereby
facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem
cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of
the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool
for fabricating controlled cellular co-cultures for cell–cell interaction studies and tissue engineering applications.
ISSN
0142-9612
Language
English
URI
http://hdl.handle.net/10371/6222
DOI
https://doi.org/10.1016/j.biomaterials.2003.10.033
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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