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A new method for rapid screening of bacterial species- or subspecies-specific DNA probes.
Cited 11 time in
Web of Science
Cited 12 time in Scopus
- Authors
- Issue Date
- 2003-02
- Citation
- FEMS Microbiol Lett. 219(1),121-7.
- Keywords
- Inverted dot blot hybridization ; Screening method ; DNA probe
- Abstract
- A simple assay for the rapid screening of bacterial species- or subspecies-specific DNA probes for the random cloning method is presented, involving the use of genomic DNAs as probes and recombinant plasmid DNAs containing genomic DNA digested with Hin dIII as targets. The optimal amount of target DNAs and the concentration of digoxigenin-labeled genomic DNA probes were 20 ng and 100 ng ml−1 (or 10 ng and 200 ng ml−1), respectively. The method was applied to the development of Fusobacterium nucleatum subspecies-specific probes. Our results showed that four out of 96 probes were F. nucleatum subspecies-specific, which was confirmed by Southern blot analysis. Our results indicate that the new method can be used for the rapid screening of species- or subspecies-specific probes.
- ISSN
- 0378-1097
- Language
- English
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