Browse

Neopterin, a marker of immune response, and 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress, correlate at high age as determined by automated simultaneous high-performance liquid chromatography analysis of human urine

Cited 30 time in Web of Science Cited 32 time in Scopus
Authors
Svoboda, Peter; Ko, Seong-Hee; Cho, Belong; Yoo, Sang-Ho; Choi, Seong-Won; Ye, Sang-Kyu; Kasai, Hiroshi; Chung, Myung-Hee
Issue Date
2008-09-30
Publisher
Elsevier
Citation
Anal Biochem. 2008 Dec 15;383(2):236-42. Epub 2008 Sep 15.
Keywords
AdultAgedAged, 80 and overAging/*immunology/metabolism/*urineAutomationBiological Markers/*urineChromatography, High Pressure LiquidDeoxyguanosine/*analogs & derivatives/urineEnzyme-Linked Immunosorbent AssayFemaleHumansMaleMiddle AgedNeopterin/*immunology/*urine*Oxidative StressSensitivity and SpecificityTime Factors
Abstract
Using an established high-performance liquid chromatography (HPLC) method based on anion exchange chromatography, fraction collection, and electrochemical detection, the oxidative DNA damage marker 8-hydroxy-2'-deoxyguanosine (8-OH-dG) can be analyzed rapidly and precisely in human urine samples. In addition, by ultraviolet (UV) detection, it was shown recently that it is possible to simultaneously analyze creatinine and 7-methylguanine (m(7)Gua), an RNA degradation product, in urine. By adding a fluorescence detector to the HPLC system, we now report that it is also possible to detect pteridins such as neopterin and biopterin. The fluorescence detection was evaluated in detail for neopterin, an immune response and tumor marker. The urinary content of neopterin, assessed by using the HPLC method, was verified with a commercial neopterin enzyme-linked immunosorbent assay (ELISA) kit as indicated by the high correlation between the two methods (r=0.98). In urinary samples from 58 young healthy individuals (male and female nonsmokers, ages 19-39 years), it was found that there was no significant correlation (r=-0.04) between the levels of 8-OH-dG and neopterin (as normalized to urinary creatinine levels). In contrast, in urinary samples from 60 old healthy individuals (male and female nonsmokers, ages 60-86 years), there was a significant correlation (r=0.47) found between the levels of 8-OH-dG and neopterin (as normalized to urinary creatinine levels). These findings strongly indicate that the higher level of immune response that was correlating with old age contributes significantly to the higher level of oxidative damage as assessed in the form of 8-OH-dG. Using this type of HPLC system, it is possible to evaluate oxidative DNA damage and immune response simultaneously using the respective urinary markers. These data may contribute to understanding of the pathophysiology of diseases such as infections and tumor progression where both oxidative stress and immune response occur simultaneously.
ISSN
1096-0309 (Electronic)
1096-0309 (Linking)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18822267

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6W9V-4TFM7KM-2-9&_cdi=6692&_user=168665&_orig=search&_coverDate=12%2F15%2F2008&_sk=996169997&view=c&wchp=dGLbVlW-zSkzS&md5=4ef37ad7335532a4d451d86f821cb2f8&ie=/sdarticle.pdf

http://hdl.handle.net/10371/62423
DOI
https://doi.org/10.1016/j.ab.2008.09.014
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Family Medicine (가정의학전공)Journal Papers (저널논문_가정의학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse