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College of Medicine/School of Medicine (의과대학/대학원)
Surgery (외과학전공)
Journal Papers (저널논문_외과학전공)
CD24 cross-linking induces apoptosis in, and inhibits migration of, MCF-7 breast cancer cells
- Issue Date
- 2008-04-25
- Publisher
- BioMed Central
- Citation
- BMC Cancer 8:118-127
- Keywords
- Animals ; Antigens, CD24/*immunology ; Apoptosis/*immunology ; Breast Neoplasms/immunology/pathology/therapy ; Cell Line, Tumor ; Complement System Proteins/*immunology/metabolism ; Feedback, Biochemical ; Female ; Humans ; Immunoglobulin Fc Fragments/metabolism/therapeutic use ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Rabbits ; Receptors, Fc/metabolism ; Signal Transduction
- Abstract
- BACKGROUND: The biological effects of CD24 (FL-80) cross-linking on breast cancer cells have not yet been established. We examined the impact of CD24 cross-linking on human breast cancer cell line MCF-7. METHODS: MCF-7 and MDA-MB-231 cells were treated with anti-rabbit polyclonal IgG or anti-human CD24 rabbit polyclonal antibodies to induce cross-linking, and then growth was studied. Changes in cell characteristics such as cell cycle modulation, cell death, survival in three-dimensional cultures, adhesion, and migration ability were assayed after CD24 cross-linking in MCF-7. RESULTS: Expression of CD24 was analyzed by flow cytometry in MDA-MB-231 and MCF-7 cells where 2% and 66% expression frequencies were observed, respectively. CD24 cross-linking resulted in time-dependent proliferation reduction in MCF-7 cells, but no reduction in MDA-MB-231 cells. MCF-7 cell survival was reduced by 15% in three-dimensional culture after CD24 cross-linking. Increased MCF-7 cell apoptosis was observed after CD24 cross-linking, but no cell cycle arrest was observed in that condition. The migration capacity of MCF-7 cells was diminished by 30% after CD24 cross-linking. CONCLUSION: Our results showed that CD24 cross-linking induced apoptosis and inhibited migration in MCF-7 breast cancer cells. We conclude that CD24 may be considered as a novel therapeutic target for breast cancer.
- ISSN
- 1471-2407 (Electronic)
- Language
- English
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- College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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