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Molecular Cloning and Functional Expression of a Sodium Bicarbonate Cotransporter from Guinea-Pig Parotid Glands
DC Field | Value | Language |
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dc.contributor.author | Park, Kyungpyo | - |
dc.contributor.author | Koo, Na-Youn | - |
dc.contributor.author | Li, Jingchao | - |
dc.contributor.author | Hwang, Sung Min | - |
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Lee, Sung Joong | - |
dc.contributor.author | Oh, Seog-Bae | - |
dc.contributor.author | Kim, Joong-Soo | - |
dc.contributor.author | Lee, Jong Heun | - |
dc.date.accessioned | 2010-04-13T01:12:35Z | - |
dc.date.available | 2010-04-13T01:12:35Z | - |
dc.date.issued | 2006-04 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 342 (2006) 1114-1122. | en |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | http://dx.doi.org/10.1016/j.bbrc.2006.02.064 | - |
dc.identifier.uri | https://hdl.handle.net/10371/63037 | - |
dc.description.abstract | We recently found that the concentration of in guinea-pig saliva is very similar to that of human saliva; however, the entity that regulates transport has not yet been fully characterized. In order to investigate the mechanism of transport, we identified, cloned, and characterized a sodium bicarbonate cotransporter found in guinea-pig parotid glands (gpNBC1). The gpNBC1 gene encodes a 1079-amino acid protein that has 95% and 96% homology with human and mouse parotid NBC1, respectively. Oocytes expressing gpNBC1 were exposed to or Na+-free solutions, which resulted in a marked change in membrane potentials (Vm), suggesting that gpNBC1 is electrogenic. Likewise, a gpNBC1-mediated pH recovery was observed in gpNBC1 transfected human hepatoma cells; however, in the presence of 4, 4-diisothiocyanostilbene-2,2-disulfonic acid, a specific NBC1 inhibitor, such changes in Vm and pHi were not observed. Together, the data show that the cloned guinea-pig gene is a functional, as well as sequence homologue of human NBC1. | en |
dc.description.sponsorship | This work was supported by a Korea Research Foundation Grant, KRF-2003-041-E20215. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | cotransporter | en |
dc.subject | Guinea-pig | en |
dc.subject | Parotid glands | en |
dc.subject | DIDS | en |
dc.title | Molecular Cloning and Functional Expression of a Sodium Bicarbonate Cotransporter from Guinea-Pig Parotid Glands | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박경표 | - |
dc.contributor.AlternativeAuthor | 고나연 | - |
dc.contributor.AlternativeAuthor | 황성민 | - |
dc.contributor.AlternativeAuthor | 최세영 | - |
dc.contributor.AlternativeAuthor | 이성종 | - |
dc.contributor.AlternativeAuthor | 오석배 | - |
dc.contributor.AlternativeAuthor | 김종수 | - |
dc.contributor.AlternativeAuthor | 이종현 | - |
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