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Gene expression of Clonorchis sinensis metacercaria induced by gamma irradiation

Cited 4 time in Web of Science Cited 4 time in Scopus
Authors
Kim, Tae Im; Cho, Pyo Yun; Song, Kwang Jin; Li, Shunyu; Hong, Sung-Jong; Park, Suk Won; Chai, Jong-Yil; Shin, Eun-Hee
Issue Date
2008-01-29
Publisher
Springer Verlag
Citation
Parasitol Res. 2008 May;102(6):1143-50. Epub 2008 Jan 27.
Keywords
AnimalsBase SequenceClonorchis sinensis/*genetics/*radiation effectsDNA, Helminth/chemistry/geneticsExpressed Sequence Tags*Gamma Rays*Gene Expression ProfilingGene LibraryGenes, HelminthLethal Dose 50Molecular Sequence DataPolymerase Chain Reaction/methodsSequence Analysis, DNAUp-Regulation
Abstract
Gamma-rays are a form of ionizing radiation and produce serious cellular damage to nuclei and organelles. Gamma irradiation induces the expressions of genes involved in DNA repair. Clonorchis sinensis resides in and provokes pathophysiologic changes in the bile ducts of mammals. The C. sinensis metacercariae are unsusceptible or resistant to gamma irradiation with LD50 of 16.5 Gy. Using the annealing control primer-based polymerase chain reaction (PCR) method, 19 genes were found to be up-regulated in C. sinensis metacercariae exposed to gamma rays. Contigs of up-regulated genes (URGs) were retrieved in a C. sinensis expressed sequence tag pool and extended by DNA-walking. Of the 13 URGs annotated putatively as functional genes, five URGs were associated with energy metabolism, six with protein processing, and the other two with DNA repair protein RAD23 and inhibitor of apoptosis protein. Four URGs were confirmed up-regulated by gamma irradiation by quantitative real-time PCR. One unknown gene, which was up-regulated to the greatest extent, might contribute to early recovery from gamma-irradiation-induced damage. The up-regulations of genes encoding DNA repair, protein processing, and energy metabolism proteins suggests that increases in gene products orchestrate DNA lesion repair and recover cellular functions in gamma-irradiated C. sinensis metacercariae.
ISSN
0932-0113 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18224473

http://www.springerlink.com/content/x27647k582552734/fulltext.pdf

http://hdl.handle.net/10371/63094
DOI
https://doi.org/10.1007/s00436-008-0882-y
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College of Medicine/School of Medicine (의과대학/대학원)Parasitology and Tropical Medicine (기생충학전공)Journal Papers (저널논문_기생충학전공)
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