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peR Amplification of DNA Extracted from Formalin-Fixed Tissue

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Authors
Lee, Jung Bin; Lee, Yoon Seong
Issue Date
1992-06
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med 1992;33(1):115-119
Keywords
Formalin-fixed tissuePCR
Abstract
In this study we try to confirm whether the DNA from formalin-fixed tissue
be suitable for forensic application such as individual identification or examination
of medicolegal evidences using PCR. Fresh placenta were taken from 18 different
women and DNA was extracted . Some portion of placenta were fixed in 10% buffered
neutral formalin for 24 hour and DNA was extracted. Two loci of DlS80 and D17S30
were used for PCR amplification. The amplification pattern were identical between fresh
tissue and formalin fixed tissue suggesting that the formalin fixed tissue can be used for
medicolegal situation. But the DNA from formalin seemed to be degraded as the fixation
time prolonged.
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/6349
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 33 No.2 (1992)
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