peR Amplification of DNA Extracted from Formalin-Fixed Tissue

Abstract

In this study we try to confirm whether the DNA from formalin-fixed tissue be suitable for forensic application such as individual identification or examination of medicolegal evidences using PCR. Fresh placenta were taken from 18 different women and DNA was extracted . Some portion of placenta were fixed in 10% buffered neutral formalin for 24 hour and DNA was extracted. Two loci of DlS80 and D17S30 were used for PCR amplification. The amplification pattern were identical between fresh tissue and formalin fixed tissue suggesting that the formalin fixed tissue can be used for medicolegal situation. But the DNA from formalin seemed to be degraded as the fixation time prolonged.