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The Effect of UVA and UVB on DNA Synthesis and Unscheduled DNA Synthesis in Mouse Skin

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Authors
Youn, Jai il; Kim, Kyu Han; Lee, Myung Chul
Issue Date
1992-06
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med 1992;33(2):141-146
Keywords
UVAUVBAutoradiographyDNA synthesisUnscheduled DNA synthesis
Abstract
The effects of ultraviolet light A (UVA) and ultraviolet light B (UVB) on
DNA synthesis and unscheduled DNA synthesis (UDS) were studied in mouse skin by
microautoradiography. The mice were exposed to 50mJ / em" UVB by fluorescent
sunlamp or 50J / ern" UVA by metal halide mercury lamp. Time course studies were
performed immediately, 6 hours, 24 hours, and 48 hours after UVA and UVB exposure.
There was no decreased number of heavily labeled cells (HLC) representing DNA synthesis
immediately after UVA exposure and up to 48 hours postirradiation. However,
immediately after and at 6 hours after UVA irradiation there was an increased number
of sparsely labeled cells (SLC) representing UDS. Recovery was noticed 24 hours after
irradiation and it was maintained after 48 hours postirradiation. These results clearly
demonstrate that UVA induces considerable DNA damage and repair. DNA synthesis
decreased immediately and at 6 hours and at 24 hours after UVB exposure. It
recovered at 48 hours after UVB exposure. UDS increased immediately and at 6 hours
after UVB exposure. Repair synthesis was completed at 24 hours after UVB exposure.
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/6353
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 33 No.2 (1992)
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