S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 29 No.2 (1988)
Purification and Characterization of Acidic Glutathione-S-Transferase from Human Peripheral Blood Leukocyte
인체 백혈구 Glutathione-S-Transferase의 정제 및 특성에 관한 연구
- Han, Jung Ho
- Issue Date
- Seoul J Med 1988;29(2):179-186
- An acidic isoenzyme of glutathione-S-transferase (Ee 188.8.131.52) has been purified
to homogeneity from human peripheral leukocytes through a combination of ion-exchange,
gel filtration and S-hexylglutathione-agarose affinity chromatography. The purified enzyme
exhibited specific activity of 188 ,u mol· min-1 . mg- 1 toward l-chloro-2,4-dinitrobenzene.
The molecular weight of the protein, as determined by gel filtration, was 48,000. Electrophoresis
under denaturing condition revealed a single band of molecular weight about
24500. indicating the enzyme is composed of two subunits with approximately equal size. The
enzyme had an isoelectric point of 4.6, and an apparent Km of 0.64 mM for
l-chloro-2,4-dinitrobenzene and 0.21 mM for reduced glutathione.
- Files in This Item: There are no files associated with this item.