Study on In vitro Fertilization of Mouse
생쥐난자의 체외수정에 관한 연구

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dc.contributor.authorSohn, Cherl-
dc.contributor.authorChang, Yoon Seok-
dc.identifier.citationSeoul J Med 1985;26(4):361-368en
dc.description.abstractThe success of human in vitro fertilization (IVF) and embryo transfer (ET) has
focused attention on the culture conditions that can provide optimal development of the
preimplantation embryo. Studies of in vitro fertilization using mouse have direct implications to
human IVF, since similar conditions are used for both species. Mouse IVF was studied with
special reference to the condition of culture media and vessels since April 1984.
The results were summarized as follows:
1. Egg retrieval after superovulation in mice was 17.14±4.79 eggs per mouse.
2. About fifty percent of eggs retrieved showed good quality which has no evidence of
fragmentation of the ooplasm or globules of unequal size.
3. In comparison with two media and two culture vessels after 72 hours in vitro culture,
cleavage rates (six to eight-cell blastocyst) were 9.7% (Ham's F-10 in dish), 10.9% (Ham's
F-10 in tube), 21.0% (m-KRB in dish), and 36.6% (m-KRB in tube) respectively.
4. Am-KRB medium was superior to Ham's F-10 in developing mouse embryo.
It was suggested that mouse IVF system has a valuable place in a quality control system of
human IVF and a research model.
dc.publisherSeoul National University College of Medicineen
dc.subjectIn Vitro Fertilizationen
dc.subjectCulture Mediaen
dc.titleStudy on In vitro Fertilization of Mouseen
dc.title.alternative생쥐난자의 체외수정에 관한 연구en
dc.typeSNU Journalen
dc.citation.journaltitle서울 의대 잡지 = 서울 의대 학술지 = Seoul Journal of Medicine-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 26 No.4 (1985)
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