Effect of Myeloperoxidase/Hydrogen Peroxide/Halide System on Thermal Gelation of Soluble Collaqen
Myeloperoxidase/H2O2/Halide 산화계에 의한 용해성 Collagen의 열성 Gel 형성에 미치는 영향

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Yoo, Hyung-Joon; Chung, Myung-Hee; Chang, Young-Joo; Kim, Yong-Sik; Lim, Jung-Kyoo
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Seoul National University College of Medicine
Seoul J Med 1987;28(2):61-71
CollagenMyeloperoxidasePolymorphonuclear leukocytes
Effect of myeloperoxidase (MPO)/H202/halide system on thermal gelation of
soluble collagen was examined to test the ability of the system to alter the structure of this
structural protein using MPO prepared from human polymorphonuclear leukocytes. The gelation
that occurred at 3rC was markedly inhibited when collagen treated with MPO, H202 and
halides. The inhibition required the three components. All the halides except F- were effective
as cofactors. The inhibition was prevented by 102 scavengers such as histidine and
diazobicyclo(2,2,2)octane. With these findings it is confirmed that the inhibition of collagen
gelation was caused by MPO-mediated peroxidation. The nature of structural alterations by
the system that resulted in the inhibition of gelation was not explored in detail. But collagen
when digested with collagenase did not gel properly and collagen treated with the MPO system
was found to be cleaved on chromatographic analysis. Thus, degradation may be one of the
changes in collagen structure caused by the MPO system.
The results obtained indicate that MPO/H202/haiide system was able to cause structural
alteration of collagen. The degradative effect of the MPO system on collagen structure was
discussed as a possible mechanism of tissue damage in inflammatory conditions.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 28 No.2 (1987)
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