Publications
Detailed Information
In vivo induction of mucosal immune responses by intranasal administration of chitosan microspheres containing Bordetella bronchiseptica DNT
Cited 46 time in
Web of Science
Cited 60 time in Scopus
- Authors
- Issue Date
- 2006-03-10
- Publisher
- Elsevier
- Citation
- Eur. J. Pharm. Biopharm. 63 (2006) 215–220
- Keywords
- Bordetella bronchiseptica ; DNT ; Chitosan microspheres ; Mucosal immunity ; Nasal administration
- Abstract
- In vitro immune-stimulating activities of Bordetella bronchiseptica dermonecrotoxin (BBD)-loaded in chitosan microspheres (CMs)
were reported with a mouse alveolar macrophage cell line (RAW264.7). Based on the report, in vivo activity of immune-induction
was investigated by intranasal administration of the BBD-loaded CMs into mice. BBD was loaded into the CMs prepared by an ionic
gelation process with tripolyphosphate. Mice were immunized by direct administration of the BBD-loaded CMs into the nasal cavity.
After immunization of the mice, BBD-specific immune responses (IgG and IgA titers) were measured in sera, nasal wash, and saliva
by ELISA. BBD-specific IgA titers in the nasal cavity were time- and dose-dependently increased by the administration. Similar phenomena
were observed in the analysis of systemic IgA and IgG in sera. However, the antibody in saliva was undetectable by ELISA. These
results suggested that direct vaccination via the nasal cavity was effective for targeting nasal-associated lymphoid tissues, and that CMs
were an efficient adjuvant in nasal mucosal immunity for atrophic rhinitis vaccine.
- ISSN
- 0939-6411
- Language
- English
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.