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In vivo induction of mucosal immune responses by intranasal administration of chitosan microspheres containing Bordetella bronchiseptica DNT

Cited 43 time in Web of Science Cited 56 time in Scopus
Authors
Kang, Mi Lan; Kang, Sang Gyun; Jiang, Hu-Lin; Shin, Seung Won; Lee, Deog Yong; Ahn, Jeong-Min; Rayamahjia, Nabin; Park, In-Kyu; Shin, Sung Jae; Cho, Chong-Su; Yoo, Han Sang
Issue Date
2006-03-10
Publisher
Elsevier
Citation
Eur. J. Pharm. Biopharm. 63 (2006) 215–220
Keywords
Bordetella bronchisepticaDNTChitosan microspheresMucosal immunityNasal administration
Abstract
In vitro immune-stimulating activities of Bordetella bronchiseptica dermonecrotoxin (BBD)-loaded in chitosan microspheres (CMs)
were reported with a mouse alveolar macrophage cell line (RAW264.7). Based on the report, in vivo activity of immune-induction
was investigated by intranasal administration of the BBD-loaded CMs into mice. BBD was loaded into the CMs prepared by an ionic
gelation process with tripolyphosphate. Mice were immunized by direct administration of the BBD-loaded CMs into the nasal cavity.
After immunization of the mice, BBD-specific immune responses (IgG and IgA titers) were measured in sera, nasal wash, and saliva
by ELISA. BBD-specific IgA titers in the nasal cavity were time- and dose-dependently increased by the administration. Similar phenomena
were observed in the analysis of systemic IgA and IgG in sera. However, the antibody in saliva was undetectable by ELISA. These
results suggested that direct vaccination via the nasal cavity was effective for targeting nasal-associated lymphoid tissues, and that CMs
were an efficient adjuvant in nasal mucosal immunity for atrophic rhinitis vaccine.
ISSN
0939-6411
Language
English
URI
http://hdl.handle.net/10371/6475
DOI
https://doi.org/10.1016/j.ejpb.2006.01.010
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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