SHERP

Molecular Typing by Random Amplification of Polymorphic DNA (RAPD) and Detection of Virulence Genes of Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum

Cited 0 time in webofscience Cited 10 time in scopus
Authors
Jin, Ji-Dong; Lee, Dong-Seok; Shin, Eun-Kyung; Kim, Sun-Joong; Jung, Rose; Hahn, Tae-Wook
Issue Date
2006
Publisher
Japanese Society of Veterinary Science
Citation
J. Vet. Med. Sci. 68: 1321-1326
Keywords
RAPDSalmonella Gallinarumuniversal rice primersvirulence gene
Abstract
Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum is the causative agent of fowl typhoid in chickens, outbreaks of which have devastated poultry populations in Korea since 1992. In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined using the random amplified polymorphic DNA (RAPD) method. Of 13 arbitrary primers screened initially, the primer designated as universal rice primer-6 (URP-6) was selected for subsequent typing assays because it produced a distinctive and reproducible DNA fingerprint for a S. Gallinarum reference strain. URP-6-based RAPD analysis assigned 30 S. Gallinarum isolates into 6 types, with 26 isolates (86.6%) belonging to 2 major RAPD types. The distribution of virulence genes in S. Gallinarum isolates was examined by Southern hybridization. All tested isolates had the invasion gene, invA, the virulence plasmid gene, spvB, and the S. Enteritidis fimbrial gene, sefC. The distribution of virulence genes among S. Gallinarum isolates did not correlate with any specific RAPD type.
ISSN
0916-7250 (print)
1347-7439 (online)
Language
English
URI
http://hdl.handle.net/10371/6493
DOI
https://doi.org/10.1292/jvms.68.1321
Files in This Item:
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse