SHERP

Intrathecal treatment with σ1 receptor antagonists reduces formalin-induced phosphorylation of NMDA receptor subunit 1 and the second phase of formalin test in mice

Cited 0 time in webofscience Cited 0 time in scopus
Authors
Kim, Hyun-Woo; Kwon, Young-Bae; Roh, Dae-Hyun; Yoon, Seo Yeon; Han, Ho-Jae; Kim, Kee-Won; Beitz, Alvin J; Lee, Jang-Hern
Issue Date
2009-01-29
Publisher
Wiley-Blackwell;
British Pharmacological Society
Citation
Br. J. Pharmacol.,148, 490–498
Keywords
σ1 receptorBD-1047BMY-14802formalin testFosNMDA phosphorylation
Abstract
1 Although previous reports have suggested that the sigma 1 (s1) receptor may be involved in pain
sensation, its specific site of action has not been elucidated. The aim of present study was to determine
the role of the spinal s1 receptor in formalin-induced pain behavior, spinal cord Fos expression and
phosphorylation of N-methyl-D-aspartate receptor subunit 1 (pNR1).
2 Intrathecal (i.t.) pretreatment with the selective s1 receptor antagonist, BD-1047 (N-[2-(3,4-
dichlorophenyl)ethyl]-N-methyl-2-(dimethylamino)ethylamine dihydrobromide) (10–100 nmol) dose
dependently reduced formalin-induced pain behaviors in second phase, but not first phase, of the
formalin test. I.t. injection of BD-1047 also reduced formalin-evoked Fos expression and pNR1 at
the protein kinase C-dependent site, serine-896 (Ser896) and the protein kinase A-dependent site,
serine-897 (Ser897) in spinal dorsal horn.
3 i.t. BMY-14802 ((a-(4-fluorophenyl)-4-(5-fluoro-2-pyrimidinyl)-1-piperazinebutanol hydrochloride)
(10–100 nmol, s1 receptor antagonist and 5-HT1A receptor agonist) dose dependently reduced
formalin-induced pain behaviors in both phases. However, the 5-HT1A receptor might not be involved
in the antinociceptive effect of BMY-14802 on the second phase, since i.t. pretreatment with the
5-HT1A receptor antagonist propranolol ((S)-1-isopropylamino-3-(1-naphthyloxy)-2-propanol hydrochloride)
(injected 10 min prior to i.t. BMY-14802) partially blocked the effect of BMY-14802 on the
first phase of the formalin test but did not affect the inhibitory effect of BMY-14802 on the second
phase. In addition, i.t. BMY-14802 significantly reduced formalin-evoked Fos expression and pNR1
(Ser896 and Ser897) expression in spinal dorsal horn.
4 The results of this study suggest that selective blockage of spinal s1 receptors can reduce pain
behaviors, spinal cord Fos expression and pNR1 (Ser896 and Ser897) expression associated with the
second phase of the formalin test.
ISSN
0007-1188
Language
English
URI
http://hdl.handle.net/10371/6522
DOI
https://doi.org/10.1038/sj.bjp.0706764
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse