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Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs

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Authors
Lee, Chul-Seung; Moon, Hyoung-Joon; Yang, Jeong Sun; Park, Seong-Jun; Song, Dae-Sub; Kang, Bo-Kyu; Park, BongKyun
Issue Date
2006-10-10
Publisher
Elsevier
Citation
J. Virol. Methods 139, 39–43
Keywords
Polymerase chain reactionPseudorabies virusPorcine cytomegalovirusPorcine circovirus
Abstract
The use of porcine organs is being developed as a means to alleviate the shortage of human organs for transplantation. Recommendations
have been published for the microbiological specifications of organ-source pigs to reduce the possibility of a microorganism from pigs being
inadvertently transferred to the recipient of the xenograft. The pseudorabies virus (PRV), porcine cytomegalovirus (PCMV), and porcine circovirus
(PCV) are infectious agents in pigs that are considered to be of significance for the microbiological safety of xenotransplantation. A multiplex
polymerase chain reaction (mPCR) was developed to detect and differentiate among PRV, PCMV, and PCV. The sensitivities of the multiplex PCR
were 102.5 TCID50/ml for PRV, 101.8 TCID50/ml for PCMV, and 101.8 TCID50/ml for PCV. The lowest viral concentrations detected by single PCR
were 101.5 TCID50/ml for PRV, 101.0 TCID50/ml for PCMV, and 101.4 TCID50/ml for PCV2. Non-specific reactions were not observed when other
viruses, bacteria, and Vero cells were used to assess the multiplex PCR. The multiplex PCR was effective in detecting various combinations of one
or more of these viruses in pig specimens collected for xenotransplantation.
ISSN
0166-0934
Language
English
URI
http://hdl.handle.net/10371/6538
DOI
https://doi.org/10.1016/j.jviromet.2006.09.003
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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