Normal Values and Comparison of Atomic Absorption Spectrophotometric and }-; luorometric Methods for Determination of Serum Magnesium

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Tchai, B. S.; Park, T. S.; Suk, Y. G.
Issue Date
서울대학교 의과대학
Seoul J Med 1971;12(3):152-156
The diagnostic implications of change in
serum magnesium levels have been evident in
recent years. 1> 2) Serum magnesium level,
however, like serum calcium, normally are
maintained within very narrow limits so that
higly accurate and specific analytical methods
are required to detect the relatively small
changes which may occur in certain disease
The magnesium content of biological fluid
has been investigated by many workers using
a variety of procedures, including magnesium
phosphate precipitation, 3) cornplexometric
titration, 4, 5) photometry, 6, 7) and emission
flame photometry. 8, 9, 10) Schachter!'? noted the
fluorescence of the magnesium compound of
S-hydroxyquinoline in ethanol and used this
property for quantitative fluorometric assay.
The fluorometric procedure possesses distinct
advantages in that it is specific, accurate,
rapid, and employs a very small sample.
Atomic absorption spectroscopy was developed
by Walsh!" as an analytical technic
applicable to the determination of many metals.
The use of atomic absorption spectroscopy for
the determination of magnesium in serum was
reported by Willis I 3, 14) in 1959 and 1960, using
direct measurements on serum diluted with an
aqueous EDTA solution. Subsequent modifica tions have involved other direct dilution methods
15-21) or protein precipitation. These published
results indicated that atomic absorption spectroscopy
would be a simple, rapid, and
practical method for the determination of
magnesium in biological material, particulary
in serum and urine, and that it could be
adapted effectvely to the needs of a clinical
chemistry laboratories.
As we required an accurate and reproducible
method for estimating serum magnesium, it
was decided to compare both procedures,
fluorometry and atomic absorption spectroscopy.
In addition, it is the purpose of this
report to complete the normal ranges of magne-sium
in serum of healthy Korean persons as
reference points evaluating pathologi c condi
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 12 No.3 (1971)
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