흰쥐 신장 MIcrosomal fraction ATPase 활성에 대한 연구

Abstract

The effects of ethacrynic acid, furosemide and ouabain on ATPase activity of rat microsomal fraction were studied in order to confirm the evidence of relation between effect on ATPase activity and their diuretic action. Ethacrynic acid and furosemide inhibit rat kidney microsomal ATPase activity and degree of their inhibitory action was dependent on K+ concentration in the medium. Ouabain shows no inhibitory action on ATPase activity but slight stimulation. Mgt t-A'I'Pase activity was inhibited by ouabain. It suggest that the inhibition of kidney microsomal ATPase activity by ethacrynic acid and furosemide will partly contribute to their diuretic action through inhibition of tubular sodium reabsorption mechanism.