각종동물조직 및 암조직의 LDH isoenzyme 및 RNase, RNase inhibitor 활성에 관한 연구

Abstract

The LDH isoenzyme and RNase activities of hepatic 'Of hepatopancreatic and muscle tissues from varying species of animals were studied. The animals examined are 1 species of echinodermate. 7 species of mollusc. 2 species of arthropod. 1 species of chordate and 6 species of vertebrate. The LDH isoenzymes and RNase activities were also examined on the parasites such as ascaris and liver fluke along with their host tissues with reference to host-parasite relationship. and the same approach was conducted with stomach and cervical cancers, comparying to their control tissues. The following results were obtained. 1) The total activities of LDH from the hepatopancreatic and muscle tissues of molluscs were extremely low and the electrophoretic patterns of LDH isoenzymes were quite different from those of the equivalent tissues of mammals. The electrophoretic separation of LDH isoenzymes in fishes. was not clear with heavy tailing. as compared to that of mammalian tissues. The specific activities of tissue LDH of animals. seem to increase as animal evolves. The specific activities of muscle LDH were generally higher than those of hepatic or hepatopancreatic tissues. 2) The RNase activity patterns of hepatopancreas and muscle of molluscs were quite different from those of equivalent tissues of mammals. It is noteworthy that the total RNase activities turned out to be much less than the free RNase activities in sea cucumber and most of molluscs. unlike higher animals. by using SH reagent(pHMB) which is well known to suppress the RNase inhibitor. Thus. SH reagent supposedly inhibited RNase activity or activated RNase inhibitor activity in lower animals in contrast with higher animals. The total RNase activities were generally higher in hepatopancreas or liver than in muscle. contrary to LDH activities. 3) The LDH isoenzymes in bovine liver fluke were electrophoretically separated into clear-cut 5 bands. in contrast with lower animals. evidencing a close host-parasite relationship. The specific activity of LDH in liver fluke. however. was very low. as other lower animals. The LDH isoenzymes in muscle of swine ascaris was clearly fractionated into 3 bands on elecrtophoresis, unlike lower animals. The total RNase activity of liver fluke was much lower than that of host liver. but the RNase inhibitor activity was similar to that of host tissue. and the activities of total RNase and RNase inhibitor in muscle of ascaris were low. It is of interest that SH reagent was proved to suppress the RNase inhibitor. contrary to lower animals. 4) The total activities of LDH and RNase inhibitor activities were higher in stomach and cervical cancer tissues than in their normal control tissues. and the electrophoretic patterns of LDH isoenzymes of these cancer tissues were slightly different from those of the control ones. The LDH-1 fraction was prominent in stomach cancer tissue. and the activity of LDH-4 fraction in cervical cancer tissue was higher than that of control group.