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The orphan nuclear receptor DAX-1 acts as a novel transcriptional corepressor of PPARgamma
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Gwang Sik | - |
dc.contributor.author | Lee, Gha Young | - |
dc.contributor.author | Nedumaran, Balachandar | - |
dc.contributor.author | Park, Yun-Yong | - |
dc.contributor.author | Kim, Kyung Tae | - |
dc.contributor.author | Park, Sang Chul | - |
dc.contributor.author | Lee, Young Chul | - |
dc.contributor.author | Kim, Jae Bum | - |
dc.contributor.author | Choi, Hueng-Sik | - |
dc.date.accessioned | 2010-06-07T01:12:42Z | - |
dc.date.available | 2010-06-07T01:12:42Z | - |
dc.date.issued | 2008-04-03 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications 370 (2008) 264-268 | en |
dc.identifier.issn | 1090-2104 (Electronic) | - |
dc.identifier.uri | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WBK-4S5V130-3-B&_cdi=6713&_user=168665&_orig=search&_coverDate=05%2F30%2F2008&_sk=996299997&view=c&wchp=dGLbVlz-zSkWA&md5=bb9ac079743a71a2e45962a8da053115&ie=/sdarticle.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/67487 | - |
dc.description.abstract | DAX-1 is an atypical nuclear receptor (NR) which functions primarily as a transcriptional corepressor of other NRs via heterodimerization. Peroxisome proliferator-activated receptor (PPAR) gamma is a ligand-dependent NR which performs a key function in adipogenesis. In this study, we evaluated a novel cross-talk mechanism between DAX-1 and PPARgamma. Transient transfection assays demonstrated that DAX-1 inhibits the transactivity of PPARgamma in a dose-dependent manner. DAX-1 directly competed with the PPARgamma coactivator (PGC)-1alpha for binding to PPARgamma. Endogenous levels of DAX-1 were significantly lower in differentiated 3T3-L1 adipocytes as compared to preadipocytes. Using a retroviral expression system, we demonstrated that DAX-1 overexpression downregulates the expression of PPARgamma target genes, resulting in an attenuation of adipogenesis in 3T3-L1 cells. Our results suggest that DAX-1 acts as a corepressor of PPARgamma and performs a potential function in the regulation of PPARgamma-mediated cellular differentiation. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | 3T3-L1 Cells | en |
dc.subject | Adipogenesis/*genetics | en |
dc.subject | Animals | en |
dc.subject | Binding, Competitive | en |
dc.subject | Cell Line | en |
dc.subject | DNA-Binding Proteins/genetics/*metabolism | en |
dc.subject | Heat-Shock Proteins/antagonists & inhibitors/metabolism | en |
dc.subject | Humans | en |
dc.subject | Mice | en |
dc.subject | PPAR gamma/*antagonists & inhibitors/metabolism | en |
dc.subject | Receptors, Retinoic Acid/genetics/*metabolism | en |
dc.subject | Repressor Proteins/genetics/*metabolism | en |
dc.subject | Transcription Factors/antagonists & inhibitors/metabolism | en |
dc.subject | Transcription, Genetic | en |
dc.subject | Transcriptional Activation | en |
dc.subject | Transfection | en |
dc.subject | Gene Expression Regulation | - |
dc.title | The orphan nuclear receptor DAX-1 acts as a novel transcriptional corepressor of PPARgamma | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김광식 | - |
dc.contributor.AlternativeAuthor | 이가영 | - |
dc.contributor.AlternativeAuthor | 박윤용 | - |
dc.contributor.AlternativeAuthor | 김경태 | - |
dc.contributor.AlternativeAuthor | 박상철 | - |
dc.contributor.AlternativeAuthor | 이영철 | - |
dc.contributor.AlternativeAuthor | 김재범 | - |
dc.contributor.AlternativeAuthor | 최흥식 | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.03.098 | - |
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