SHERP

Identification of a novel genetic locus involved in the regulation of ptsG expression in Escherichia coli
대장균의 ptsG expression 조절에 관여하는 새로운 유전자의 탐색

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Authors
이상미
Advisor
유상렬
Issue Date
2005
Publisher
서울대학교 대학원
Keywords
Escherichia coliPTSptsGrandom mutagenesisyhjVDNA microarray
Description
Thesis(master`s)--서울대학교 대학원 :농생명공학부,2005.
Abstract
The phosphoenolpyruvate-dependent carbohydrate phosphotransferase system (PTS) is responsible
for the simultaneous transfer and phosphorylation of various carbon sources in Escherichia coli.
The ptsG gene encoding enzyme IICBGlc, the membrane component of the glucose-specific PTS, is
repressed by Mlc and activated by CRP?cAMP complex. In addition, it has been reported that
various other factors are involved in the ptsG regulation such as heat shock RNA polymerase, Fis,
FruR, ArcA and mRNA degradation. In an attempt to discover a novel gene taking part in the
regulation of ptsG, a mutant whose ptsG transcription decreased in the presence of glucose
compared with the wild type strain was selected after random mutagenesis using transposon Tn10.
As a result of sequencing the PCR product amplified with a random primer annealing to the
chromosome in the vicinity of the transposon and a transposon-specific primer, yhjV was found to
bear the transposon. Furthermore, global gene expression in the mutant was monitored employing
DNA microarray technology to understand its function related to the ptsG regulation. It has been
found that ptsG expression decreased in spite of the factors contributing to the activation of
ptsG such as the promotion of unphosphorylation of EIICBGlc and derepression of Mlc stemmed from
reduced PEP and down-regulation of ArcA, one of the repressors of ptsG. These findings strongly
suggest that YhjV acts as an activator of ptsG.
Language
English
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000051542

http://hdl.handle.net/10371/67554
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Theses (Master's Degree_식품·동물생명공학부)
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