S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Identification and characterization of salicylic acid carboxyl methyltransferase gene in rice
- Issue Date
- 서울대학교 대학원
- 애기장대; Arabidopsis (arabidopsis thaliana); 메칠살리실산 (Methyl salicylate); Mesa (methyl salicylate); SAMT (SAM:salicylic acid carboxyl methyltransferase); Samt (sam:salicylic acid carboxyl methyltransferase); pathogen-related gene 1 (PR1); Pathogen-related gene 1 (pr1); 형질전환체; Transgenic plants
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004.
- Methyl esters such as methyl jasmonate, methyl salicylate, and methyl benzoate constitute
volatile floral scent, and some of them are known to act as important cellular regulators
mediating diverse responses. Methyltransferase catalyze the formation of methyl esters using
carboxylic acid-bearing substrates as methyl acceptors. Through the NCBI rice genome database
search, we identified a rice gene highly homologous to Arabidopsis jasmonic acid carboxyl
methyltransferase gene. Corresponding cDNA clone (OsSAMT) was obtained from the MAFF Gene Bank
in Japan. The cDNA was expressed in E. coli was examined enzymatic characteristics of the
protein product. The expressed protein exhibits SAMT (SAM:salicylic acid carboxyl
methyltransferase) and BAMT (SAM:benzoic acid carboxyl methyltransferase) activities in vitro.
Furthermore, the transgenic Arabidopsis overexpressing the gene was generated to study its
physiological function(s). The transgenic plant contained about 2-fold enhanced level of methyl
salicylate compared to wild type. In this transgenic plants, expression of PR1 that are known as
a response to salicylic acid was not induced by salicylic acid. These data suggest that
salicylic acid is the active form and MeSA mainly act as airborne signal in plants defense.
Recently, Feng Chen et al. observed that AtBSMT1 induced by mehyl jasmonate. OsSAMT might
participated in the antagonistic effect of jasmonate on the salicylate signaling.
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