Production of Monoclonal Antibodies Against Human Colon Cancer Cell Line(SNU-C1) and Study of the Antigen.

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Jeong, Jae Min; Lee, Sang Sup; Koh, Chang-Soon
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Seoul National University College of Medicine
Seoul J Med 1995;36(1):15-21
colon cancer antigenmonoclonal antibodycarbohydrate antigen
To study human colon cancer antigens, monoclonal antibodies were
produced by immunizing balb/c mice with colon cancer cells(SNlJ-CI, originated
from a Korean colon cancer patient). Among these monoclonal antibodies, one of
IgM type antibody 7E02 was selected for further study. The 7E02 was proved to be
effective for immunohistological staining of colorectal cancer tissues. After
purification by ammonium sulfate fractionation and Sephacryl S-3OO gel filtration,
7E02 was labeled by 1-125. Using the radiolabeled 7E02, its dissociation constants
(Kd) against SNlJ-Cl and SNlJ-C4 were measured by Scatchard plotting, and found
to be 10.7 nM and 7.1 nM, respectively. When SNlJ-CI was treated with 10 mM
sodium periodate, 7E02 was not bound to the cell surface. However, SNlJ-CI
preserved the activity to bind to 7E02 after N-acetyl neuraminidase treatment. With
these results, we could conclude that the 7E02 binds to carbohydrate antigen that
exists on the surface of colon cancer cell and excreted mucin. The antigen was
proved not to contain terminal sialic acid.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 36 No.1 (1995)
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